CD24 Is a Novel Predictor for Poor Prognosis of Hepatocellular Carcinoma after Surgery
CD24 Is a Novel Predictor for Poor Prognosis of Hepatocellular Carcinoma after Surgery
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DOI：
10.1158/1078-0432.ccr-09-0151
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发表时间：
2009-09-01
影响因子：
11.5
通讯作者：
Fan, Jia
中科院分区：
文献类型：
--
作者：
Yang, Xin-Rong;Xu, Yang;Fan, Jia
目的：为探讨CD 24在肝细胞癌（HCC）侵袭和预后中的作用，采用实时荧光定量PCR和Western blot方法检测了CD 24在逐步转移的HCC细胞系、肿瘤、癌旁组织和正常肝组织中的表达。CD 24在HCC中的作用通过使用小干扰RNA的CD 24去除来研究。采用1997年至2000年期间314例接受切除术的HCC患者的肿瘤组织微阵列检测CD 24、P-连环蛋白和增殖细胞核抗原的表达。使用Kaplan-Meier生存估计和log-rank tests.Results：CD 24在高转移性肝癌细胞系和复发性肝癌患者的肿瘤组织中过表达。在体外，CD 24的消耗引起细胞增殖、迁移和侵袭力的显著降低。单变量和多变量分析显示，CD 24是总生存率和无复发生存率的重要预测因子。CD 24表达与预后不良相关，与甲胎蛋白、肿瘤淋巴结转移分期和Edmondson分期无关。CD 24高表达与β-catenin胞浆和胞核积聚（P = 0.023）、高肿瘤增殖状态（P = 0.018）、弥漫性肝内复发和远处转移（P = 0.026）显著相关。术后辅助性经导管动脉化疗栓塞降低了早期复发率（
Purpose: To investigate the role of CD24 in tumor invasion and prognostic significance in hepatocellular carcinoma (HCC).Experimental Design: CD24 expression was measured in stepwise metastatic HCC cell lines, tumor, peritumoral tissues, and normal liver tissues by quantitative real-time PCR and Western blot. The role of CD24 in HCC was investigated by CD24 depletion using small interfering RNA. Tumor tissue microarrays of 314 HCC patients who underwent resection between 1997 and 2000 were used to detect expression of CD24, P-catenin, and proliferating cell nuclear antigen. Prognostic significance was assessed using Kaplan-Meier survival estimates and log-rank tests.Results: CD24 was overexpressed in the highly metastatic HCC cell line and in tumor tissues of patients with recurrent HCC. Depletion of CD24 caused a notable decrease in cell proliferation, migration, and invasiveness in vitro. Univariate and multivariate analyses revealed that CD24 was a significant predictor for overall survival and relapse-free survival. CD24 expression was correlated with poor prognosis independent of a-fetoprotein, tumor-node-metastasis stage, and Edmondson stage. High CD24 expression was significantly associated with cytoplasmic and nuclear accumulation of beta-catenin (P = 0.023), high tumor proliferative status (P = 0.018), and diffused intrahepatic recurrence and distant metastasis (P = 0.026). Adjuvant transcatheter arterial chemoembolization after surgery reduced the rate of early recurrence (