The HTLV receptor is a widely expressed protein.
The HTLV receptor is a widely expressed protein.
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HTLV 受体是一种广泛表达的蛋白质。
DOI：
10.1006/viro.2000.0143
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Ratner,L
中科院分区：
文献类型：
--
作者：
Trejo,SR;Ratner,L
人类 T 细胞白血病病毒 1 型 (HTLV-1) 的受体被发现在来自多个物种的多种细胞系中表达。使用人类免疫缺陷病毒 1 型颗粒评估受体表达，用 HTLV-1 包膜糖蛋白进行假型化，并在 SV40 增强子和启动子的控制下表达荧光素酶。假型病毒的感染被 HTLV-1 的中和抗体阻断，并且感染依赖于包膜蛋白前体中切割和融合序列的存在。易感靶淋巴细胞的胰蛋白酶处理减少了进入。入口对氯化铵有部分抵抗力。
The receptor for human T-cell leukemia virus type 1 (HTLV-1) was found to be expressed on a broad range of cell lines derived from multiple species. Receptor expression was assessed using human immunodeficiency virus type 1 particles, pseudotyped with the HTLV-1 envelope glycoprotein, and expressing luciferase under the control of an SV40 enhancer and promoter. Infection by pseudotyped virus was blocked with neutralizing antibodies to HTLV-1, and infection was dependent on the presence of the cleavage and fusogenic sequences in the envelope protein precursor. Trypsin treatment of susceptible target lymphocytes reduced entry. Entry was partially resistant to ammonium chloride.