Inhibitory effect of Nordy on HPV16 E6 gene in human immortalized endocervical cells
Inhibitory effect of Nordy on HPV16 E6 gene in human immortalized endocervical cells
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Nordy对人永生化宫颈管细胞HPV16 E6基因的抑制作用
DOI：
10.1007/s11670-008-0001-8
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发表时间：
2008-03-01
影响因子：
5.1
通讯作者：
Bian Xiu-wu
中科院分区：
文献类型：
--
作者：
Chen Wei;Zhao Yong;Bian Xiu-wu
目的：研究诺地对人宫颈永生化细胞hpv16e6基因的抑制作用。方法：采用免疫细胞化学法检测H8细胞E6、p53蛋白表达，RT-PCR法检测H8细胞HPV16E6 mRNA表达。结果：免疫细胞化学结果显示，100 μ mol/L Nordy作用72 h后H8细胞E6、p53蛋白表达明显弱于对照组。经50、100、200 μ mol/L Nordy处理72 h后，H8细胞HPV16E6 mRNA表达量显著低于对照组(P
Objective: To study the inhibitory effect of Nordy on HPV16 E6 gene in human immortalized endocervical cells (H8). Methods: The expressions of E6 and p53 proteins of H8 cells were detected by immunocytochemistry, and the expression of HPV16E6 mRNA of H8 cells was detected by RT-PCR. Results: According to immunocytochemistry, expressions of E6 and p53 proteins of H8 cells after treatment with 100 mu mol/L Nordy for 72 h were weaker than control group obviously. After treatment with 50, 100, 200 mu mol/L Nordy for 72 h. HPV16E6 mRNA expressions of H8 cells were lower than the control group significantly (P