ANTIOXIDANT ROLE OF RHODNIUS-PROLIXUS HEME-BINDING PROTEIN - PROTECTION AGAINST HEME-INDUCED LIPID-PEROXIDATION
ANTIOXIDANT ROLE OF RHODNIUS-PROLIXUS HEME-BINDING PROTEIN - PROTECTION AGAINST HEME-INDUCED LIPID-PEROXIDATION
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DOI：
10.1074/jbc.270.18.10893
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发表时间：
1995-05-05
影响因子：
4.8
通讯作者：
OLIVEIRA, PL
中科院分区：
文献类型：
--
作者：
DANSAPETRETSKI, M;RIBEIRO, JMC;OLIVEIRA, PL
水溶液中的血红素积极促进导致生物分子降解的自由基反应。吸血昆虫Rhodnius prolixus在其血淋巴中具有血红素结合蛋白（RHBP）（Oliveira，P.L.，Kawooya，J .K，Ribeiro，J. M. C.的方法，Meyer，T.，普尔曼河，Alves，E，W.，步行者，F.，帕多万湾J.，和增田，H.（1994）J，Biol，Chem，270，10897-10901，在此，我们表明该蛋白质抑制亚麻酸脂质体和载脂蛋白（昆虫血淋巴的主要脂蛋白）两者的血红素依赖性过氧化。在其装载来自脂肪体的磷脂的能力以及将磷脂递送至生长的在卵母细胞中，RHBP防止血红素诱导的脂质运载蛋白的氧化损伤。有人提出，在体内RHBP结合血红素来源于血液血红蛋白的消化，抑制活性氧的产生，并保护昆虫在整个饲养周期中免受氧化应激。
Heme in aqueous solutions actively promotes free radical reactions leading to degradation of biological molecules, The blood-sucking insect Rhodnius prolixus has a heme-binding protein (RHBP) in its hemolymph (Oliveira, P. L., Kawooya, J .K, Ribeiro, J. M. C., Meyer, T., Poorman, R., Alves, E, W., Walker, F., Padovan, G. J., and Masuda, H. (1994) J, Biol, Chem, 270, 10897-10901, Here we show that this protein inhibits heme-dependent peroxidation of both linolenic acid liposomes and lipophorin, the main lipoprotein of insect hemolymph, The oxidized lipophorin is functionally impaired, being defective both in its capacity to be loaded with phospholipids from the fat body as well as in its ability to deliver phospholipids to the growing oocytes, RHBP prevents the heme-induced oxidative damage to lipophorin. It is proposed that in vivo RHBP binds the heme derived from digestion of blood hemoglobin, suppressing the generation of activated oxygen species and protecting the insect against oxidative stress throughout the feeding cycle.