Chinese Herbal Prescription QYSL Prevents Progression of Lung Cancer by Targeting Tumor Microenvironment
Chinese Herbal Prescription QYSL Prevents Progression of Lung Cancer by Targeting Tumor Microenvironment
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DOI：
10.32604/oncologie.2022.022116
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发表时间：
2022-01-01
期刊：
影响因子：
0.9
通讯作者：
Li, Ping
中科院分区：
文献类型：
--
作者：
Chen, Yang;Wu, Huan;Li, Ping
目的：肺癌是成人常见的恶性肿瘤，居中国五大恶性肿瘤的发病率和死亡率之首。我们以往的研究表明，清肺灵方能平衡肺癌小鼠Th1/Th2，抑制肿瘤细胞免疫逃逸。在此，我们研究了清肺舒对肺癌相关巨噬细胞的作用及其可能的相关机制。方法：将Lewis肺癌细胞接种于C57BL/6小鼠体内，给予清肺益气汤治疗。采用流式细胞仪、RT-PCR、免疫印迹等方法检测清抑灵方对肿瘤免疫微环境的影响。结果：QYSL对肺癌小鼠的肿瘤生长有抑制作用。进一步研究表明QYSL通过促进T细胞活化，促进巨噬细胞向M1型极化，从而抑制肺癌的生长。结果表明，清肝益气汤能明显抑制M2巨噬细胞相关蛋白的表达，促进M1巨噬细胞相关蛋白的表达。此外，QYSL组STAT6和MTOR表达降低。结论：QYSL通过促进T细胞活化和M2巨噬细胞对M1的极化而有效地抑制肺癌的生长，该作用与STAT6和MTOR信号通路有关。
Objectives: Lung cancer is a common and malignant tumor in adults and ranks first in the incidence and mortality of the top five malignant tumors in China. Our previous studies have shown that QYSL prescription can balance lung cancer mice Th1/Th2 and inhibit tumor cell immune escape. Here, we examined the effects of QYSL on lung cancer associated macrophage and the potential associated mechanism. Methods: C57BL/6 mice were injected with Lewis lung cancer cells and treated with QYSL. FACS, RT-PCR, and western blot were used to examined the effect of QYSL on tumor immune microenvironment. Results: We found QYSL inhibited tumor growth in mice with lung cancer. Further study indicated that QYSL inhibited growth of lung tumor by promoting T cell activation and promoting macrophage polarization to M1 type. We found QYSL could markedly inhibit M2 macrophage related protein expression and promote M1 macrophage related protein expression. Additionally, STAT6 and MTOR expression were reduced in QYSL group. Conclusions: Overall, this study indicated that QYSL potently inhibited the growth of lung cancer by promoting T cell activation and M2 macrophage polarization to M1, which was found to be associated with the STAT6 and MTOR signaling pathway.