Direct cryogenic-temperature transmission electron microscopy imaging of phospholipid aggregates in soybean oil
Direct cryogenic-temperature transmission electron microscopy imaging of phospholipid aggregates in soybean oil
复制标题
DOI：
10.1006/jcis.2002.8229
复制
发表时间：
2002-05-01
影响因子：
9.9
通讯作者：
Talmon, Y
中科院分区：
文献类型：
--
作者：
Danino, D;Gupta, R;Talmon, Y
低温透射电子显微镜（cryo-TEM）进行了微调，使其适用于天然油为基础的系统的研究。虽然液体乙烷，冷冻剂的标本制备的选择，被发现溶解的油，我们观察到，在许多天然油中发现的甘油三酯作为冷冻保护剂，允许玻璃化的这种油系统在液氮中，冷冻剂，提供相对较低的冷却速率。使用改进的技术结合数字成像的冷却CCD相机，我们能够直接可视化反胶束和液晶聚集体形成的大豆油/大豆磷脂/水/己烷系统，这是商业上重要的大豆油加工。该方法可广泛应用于其他有机溶剂体系。(C)2002 Elsevier Science（美国）。
Cryogenic-temperature transmission electron microscopy (cryo-TEM) was fine-tuned to make it applicable to the study of a natural oil-based system. While liquid ethane, the cryogen of choice for specimen preparation, was found to dissolve the oil, we observed that the triglycerides found in many natural oils serve as cryoprotectants that allow vitrification of such oil systems in liquid nitrogen, a cryogen that provides relatively low cooling rates. Using the modified technique combined with digital imaging by a cooled CCD camera, we were able to directly visualize inverse micellar and liquid-crystalline aggregates formed in the soybean oil/soybean phospholipids/water/hexane system, which is commercially important in soybean oil processing. The method developed here should be widely applicable to other organic solvent-based systems. (C) 2002 Elsevier Science (USA).