Direct determination of ciprofloxacin by mass spectrometry after a two-step solid-phase extraction using a molecularly imprinted polymer
Direct determination of ciprofloxacin by mass spectrometry after a two-step solid-phase extraction using a molecularly imprinted polymer
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DOI：
10.1002/jssc.200500439
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发表时间：
2006-06-01
影响因子：
3.1
通讯作者：
Borrull, Francesc
中科院分区：
文献类型：
--
作者：
Caro, Ester;Marce, Rosa M.;Borrull, Francesc
以环丙沙星（CIPRO）为模板分子，采用非共价合成法制备了分子印迹聚合物（MIP）。在其用作SPE中的吸附剂之前，通过色谱法对MIP进行评价，以确认其确实是分子印迹的。然后使用MIP从尿样中选择性地提取CIPRO，通过两步SPE程序，其中商业Oasis柱和分子印迹SPE柱串联组合。这种方法可以有效去除样品中存在的基质化合物。与原始样品相比，应用该两步SPE程序后获得的尿液提取物相对干净，这使得可以将提取物直接注入质谱仪，从而以低水平定量尿液样品中的CIPRO并减少分析时间。
A molecularly imprinted polymer (MIP) has been prepared for the first time with ciprofloxacin (CIPRO) as the template molecule, via a noncovalent synthetic procedure. Prior to its use as a sorbent in SPE, the MIP was evaluated chromatographically to confirm that it was indeed molecularly imprinted. The MIP was then used to extract CIPRO selectively from urine samples by means of a two-step SPE procedure in which a commercial Oasis cartridge and a molecularly imprinted SPE cartridge were combined in series. This approach allowed the matrix compounds present in the samples to be removed effectively. The urine extracts obtained after this two-step SPE procedure was applied were relatively clean compared to the original samples, and this made it possible to inject directly the extracts into a mass spectrometer and thus quantify CIPRO in urine samples at low levels and reduce the time of analysis.