Bladder dysfunction in a new mutant mouse model with increased superoxide--lack of nitric oxide?
Bladder dysfunction in a new mutant mouse model with increased superoxide--lack of nitric oxide?
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DOI：
10.1016/j.juro.2009.09.074
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发表时间：
2010-02
期刊：
影响因子：
--
通讯作者：
Andersson KE
中科院分区：
文献类型：
--
作者：
Soler R;Füllhase C;Lu B;Bishop CE;Andersson KE
一氧化氮介导了尿道平滑肌的松弛，也可能参与了逼尿肌活动的控制。带有Immp21基因突变的小鼠体内的超氧离子水平较高，从而导致一氧化氮的生物利用量下降。我们在这个小鼠模型中研究了膀胱功能。研究对象为4~6月龄的年轻雄性突变者、18个月龄的老年雌性突变者和WT年龄匹配的健康对照。在器官浴中的离体逼尿肌条上，测定逼尿肌条对氨基甲胆碱和电场刺激的逼尿肌收缩反应。清醒动物活体膀胱测压评价膀胱功能。年轻雄性突变体的排尿显著低于对照组，排尿后残余尿量显著高于对照组。他们在开始排尿时表现出明显的排尿困难和紧张。突变小鼠和WT小鼠对氨基甲胆碱和电场刺激的逼尿肌收缩反应相似。老年雌性突变小鼠的膀胱容量和尿量低于WT对照组，排尿频率和膀胱/体重比高于WT对照组。在体外研究中，突变的逼尿肌条对氨基甲胆碱的最大反应较低。Immp21基因突变的小鼠有膀胱功能障碍，主要特征是年轻雄性的排空异常和老年雌性的逼尿肌活动增加。年轻男性逼尿肌功能保留，老年女性逼尿肌功能受损。这些动物是氧化应激的自然模型，生物可利用的一氧化氮含量较低。因此，它们是评估这些情况在膀胱功能障碍中的作用的有趣工具。
Nitric oxide mediates urethral smooth muscle relaxation and may also be involved in detrusor activity control. Mice with mutation in the Immp2l gene have high superoxide ion levels and a consequent decrease in the bioavailable amount of nitric oxide. We studied bladder function in this mouse model. Young male mutants at ages 4 to 6 months, old female mutants at age 18 months and healthy WT age matched controls were used. The detrusor contractile response to carbachol and electrical field stimulation was tested in isolated detrusor strips in organ baths. In vivo bladder function was evaluated by cystometry in conscious animals. Young male mutants had significantly lower micturition and higher post-void residual volume than WT controls. They had pronounced voiding difficulty and strained when initiating micturition. Detrusor contractile responses to carbachol and electrical field stimulation were similar in mutant and WT mice. Old female mutant mice had lower bladder capacity and micturition volume, and higher micturition frequency and bladder-to-body weight ratio than WT controls. In the in vitro study detrusor strips from mutants showed a lower maximum response to carbachol. Mice with mutation in the Immp2l gene have bladder dysfunction, mainly characterized by emptying abnormalities in young males and increased detrusor activity in old females. Detrusor function was preserved in young males and impaired in old females. These animals are a natural model of oxidative stress with low bioavailable nitric oxide. Thus, they are interesting tools in which to evaluate the role of these conditions on bladder dysfunction.
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影响因子：
2.1
作者：
Beshay, E;Carrier, S
通讯作者：
Carrier, S
影响因子：
10.3
作者：
Gomez-Pinilla, Pedro J.;Gomez, Maria F.;Pozo, Maria J.
通讯作者：
Pozo, Maria J.
影响因子：
4.5
作者：
Masuda, Hitoshi;Kihara, Kazunori;Yoshimura, Naoki
通讯作者：
Yoshimura, Naoki
影响因子：
7.3
作者：
PERSSON, K;IGAWA, Y;ANDERSSON, KE
通讯作者：
ANDERSSON, KE
影响因子：
2
作者：
de Jongh, Rik;Haenen, Guido R. M. M.;van Kerrebroeck, Philip E. V.
通讯作者：
van Kerrebroeck, Philip E. V.