Dorsoventral patterning in the Drosophila central nervous system:: the vnd homeobox gene specifies ventral column identity
Dorsoventral patterning in the Drosophila central nervous system:: the vnd homeobox gene specifies ventral column identity
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DOI：
10.1101/gad.12.22.3603
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发表时间：
1998-11-15
影响因子：
10.5
通讯作者：
Mellerick, DM
中科院分区：
文献类型：
--
作者：
McDonald, JA;Holbrook, S;Mellerick, DM
果蝇中枢神经系统由腹侧、中间和背侧三列神经外胚层细胞发育而成。在这篇文章和随附的文章中，我们研究了两个同源框基因vnd和ind在果蝇中枢神经系统内建立腹侧和中间细胞命运中的作用。在神经发生的早期，VND蛋白仅限于腹柱神经外胚层和神经母细胞，后来在复杂的神经元模式中被检测到。我们使用区分腹柱、中柱和背柱神经外胚层和神经母细胞的分子标记，以及用于选定的神经母细胞的细胞谱系标记，来表明VND的丢失将腹柱身份转变为中柱身份，并且特定的腹柱神经母细胞无法形成。相反，异位VND产生从中间柱到腹柱的转变。因此，在果蝇中枢神经系统中，VND是诱导腹侧命运和抑制中间命运的必要条件和充分条件。VND的脊椎动物同源物(Nkx2.1和2.2)在腹侧CNS中也有类似的表达，这提出了CNS中的DV模式在进化上是保守的可能性。
The Drosophila CNS develops from three columns of neuroectodermal cells along the dorsoventral (DV) axis: ventral, intermediate, and dorsal. In this and the accompanying paper, we investigate the role of two homeobox genes, vnd and ind, in establishing ventral and intermediate cell fates within the Drosophila CNS. During early neurogenesis, Vnd protein is restricted to ventral column neuroectoderm and neuroblasts; later it is detected in a complex pattern of neurons. We use molecular markers that distinguish ventral, intermediate, and dorsal column neuroectoderm and neuroblasts, and a cell lineage marker for selected neuroblasts, to show that loss of vnd transforms ventral into intermediate column identity and that specific ventral neuroblasts fail to form. Conversely, ectopic vnd produces an intermediate to ventral column transformation. Thus, vnd is necessary and sufficient to induce ventral fates and repress intermediate fates within the Drosophila CNS. Vertebrate homologs of vnd (Nkx2.1 and 2.2) are similarly expressed in the ventral CNS, raising the possibility that DV patterning within the CNS is evolutionarily conserved.