The intrinsic migratory capacity of memory T cells contributes to their accumulation in rheumatoid synovium.
The intrinsic migratory capacity of memory T cells contributes to their accumulation in rheumatoid synovium.
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记忆 T 细胞的内在迁移能力有助于其在类风湿滑膜中的积累。
DOI：
10.1002/art.1780351206
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发表时间：
1992
影响因子：
--
通讯作者：
Lipsky,PE
中科院分区：
文献类型：
--
作者：
Cush,JJ;Pietschmann,P;Oppenheimer-Marks,N;Lipsky,PE
目的：控制T细胞向类风湿滑膜渗透的机制尚未完全确定。这些研究旨在探讨T细胞表型与迁移能力的关系，以阐明T细胞在炎症部位聚集的可能机制。方法：通过对类风湿滑膜和外周血T细胞的双重免疫荧光FACS(荧光激活细胞分类器)分析，研究体内迁移细胞的特征。结果：类风湿性关节炎(RA)和正常人移行的T细胞分别为CD45RA-、CD29bright、CD11abright、L-选择素-、CD54+和CD58+。然而，迁移性RA PBTL(与正常PBTL相比)显著富含活化的HLA-DR+T细胞。RA滑膜组织淋巴细胞具有相似的表型，但其表面密度降低，而HLA-DR和VLA-1表达增加。RA滑膜组织淋巴细胞的迁移能力是正常和RA PBTL的2-3倍。结论这些研究证实了表达某种记忆表型(CD29bright、CD11abright和CD58+)的CD4+T细胞固有的迁移能力。此外，CD54+和L-选择素-的CD_4+T细胞的跨内皮迁移能力增强。这些研究表明，循环淋巴细胞的迁移模式可能与其表面表型有关，记忆T细胞的内在迁移能力是其在类风湿滑膜中聚集的原因之一。
Objective.Mechanisms controlling the infiltration of T cells into rheumatoid synovium have not been fully characterized. These studies were undertaken to investigate the relationship between T cell phenotype and migratory capacity, so as to elucidate mechanisms that might contribute to the accumulation of T cells at inflammatory sites.Methods.The characteristics of in vivo migrating cells were studied by dual‐immunofluorescence FACS (fluorescence‐activated cell sorter) analysis of rheumatoid synovial and peripheral blood T cells. Migratory cells were also characterized using a recently developed in vitro assay, wherein peripheral blood T lymphocytes (PBTL) with the capacity to migrate through endothelial cell monolayers were retrieved and assessed.Results.Migratory CD4+ T cells from rheumatoid arthritis (RA) and normal individuals were characterized as being CD45RA‐, CD29bright, CD11abright, L‐selectin‐, CD54+, and CD58+. Migrating RA PBTL (compared with normal PBTL), however, were significantly enriched in activated HLA—DR+ T cells. RA synovial tissue lymphocytes exhibited a similar phenotype, but with decreased surface density of CD4 and an increase in HLA‐DR and VLA‐1. RA synovial lymphocytes exhibited a 2–3‐fold increase in migratory capacity over normal and RA PBTL.Conclusion.These studies demonstrate the inherent migratory proficiency of CD4+ T cells that express a memory phenotype (CD29bright, CD11abright, and CD58+). In addition, enhanced transendothelial migration was observed for CD4+ T cells that were CD54+ and L‐selectin—. These studies demonstrate that the migratory patterns of circulating lymphocytes may be correlated with their surface phenotype and that the intrinsic migratory capacity of memory T cells is one component contributing to their accumulation in the rheumatoid synovium.