A NIGT1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in Arabidopsis.
A NIGT1-centred transcriptional cascade regulates nitrate signalling and incorporates phosphorus starvation signals in Arabidopsis.
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DOI：
10.1038/s41467-018-03832-6
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发表时间：
2018-04-10
影响因子：
16.6
通讯作者：
Yanagisawa S
中科院分区：
文献类型：
--
作者：
Maeda Y;Konishi M;Kiba T;Sakuraba Y;Sawaki N;Kurai T;Ueda Y;Sakakibara H;Yanagisawa S
硝酸盐是一种营养信号，可触发转录网络的复杂调控，从而调节植物的营养依赖性生长和发育。这包括硝酸盐相关基因表达的时间和硝酸盐浓度依赖性调节。然而，对潜在的机制仍然知之甚少。在这里，我们确定NIGT 1转录抑制因子作为拟南芥NRT2.1硝酸盐转运蛋白基因的负调控因子，并显示NLP初级转录因子对硝酸盐信号传导和NLP-NIGT 1转录级联介导的抑制的拮抗调控。这种拮抗性调节为硝酸盐诱导的转录调节的复杂性提供了解决方案。全基因组分析表明，这一机制适用于NRT2.1和其他参与硝酸盐同化、激素生物合成和转录的基因。此外，磷饥饿反应的PHR 1主调节因子也直接促进NIGT 1家族基因的表达，导致硝酸盐吸收减少。因此，NIGT 1阻遏物在两个转录级联中起作用，在磷和氮营养调节之间形成直接联系。植物通过调节基因表达对养分作出反应。在这里，作者表明，硝酸盐通过NIGT 1转录抑制因子抑制NRT 2.1硝酸盐转运蛋白表达，磷酸盐饥饿通过PHR 1增强这一途径，从而将磷和氮信号联系起来。
Nitrate is a nutrient signal that triggers complex regulation of transcriptional networks to modulate nutrient-dependent growth and development in plants. This includes time- and nitrate concentration-dependent regulation of nitrate-related gene expression. However, the underlying mechanisms remain poorly understood. Here we identify NIGT1 transcriptional repressors as negative regulators of the Arabidopsis NRT2.1 nitrate transporter gene, and show antagonistic regulation by NLP primary transcription factors for nitrate signalling and the NLP-NIGT1 transcriptional cascade-mediated repression. This antagonistic regulation provides a resolution to the complexity of nitrate-induced transcriptional regulations. Genome-wide analysis reveals that this mechanism is applicable to NRT2.1 and other genes involved in nitrate assimilation, hormone biosynthesis and transcription. Furthermore, the PHR1 master regulator of the phosphorus-starvation response also directly promotes expression of NIGT1 family genes, leading to reductions in nitrate uptake. NIGT1 repressors thus act in two transcriptional cascades, forming a direct link between phosphorus and nitrogen nutritional regulation. Plants respond to nutrients by modulating gene expression. Here, the authors show that nitrate suppresses NRT2.1 nitrate transporter expression via NIGT1 transcriptional repressors and that phosphate starvation enhances this pathway via PHR1, thus linking phosphorus and nitrogen signalling.
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