Enhanced intraarticular free radical reactions in adjuvant arthritis rats
Enhanced intraarticular free radical reactions in adjuvant arthritis rats
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DOI：
10.1080/10715760500440536
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发表时间：
2006-05-01
影响因子：
3.3
通讯作者：
Utsumi, H
中科院分区：
文献类型：
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作者：
Yamada, KI;Nakamura, T;Utsumi, H
类风湿性关节炎（RA）发生的原因之一是自由基反应与组织损伤的关系。本研究采用活体电子自旋共振(ESR)/硝基自旋探针技术，探讨佐剂性关节炎（AA）模型大鼠体内自由基反应增强的部位。静脉注射自旋探针后的信号衰减在AA组较对照组增强，在地塞米松（DXT）预处理后抑制。有趣的是，关节腔的衰减发生在AA足跖肿胀之前，并被同时注射自由基清除剂抑制，表明AA大鼠关节腔的自由基反应增强。该技术将为确定自由基增强反应的位置和评估抗氧化药物的活性提供无创实时测量的有用工具。
One of the reasons of rheumatoid arthritis (RA) development is widely recognized the relation of free radical reactions in tissue injuries. The aim of this study was to evaluate the location where in vivo free radical reactions was enhanced in adjuvant arthritis (AA) model rats using in vivo electron spin resonance (ESR)/nitroxyl spin probe technique. The signal decay after intravenous injection of spin probe was enhanced in AA than that in control and suppressed by the pre-treatment of dexamethasone (DXT). Interestingly, the decay in joint cavity occurred prior to paw swelling of AA and suppressed by a simultaneous injection of free radical scavengers, indicating that the enhancement of free radical reactions in joint cavity of AA rats. This technique would be useful tool to determine the location of the enhanced free radical reactions and evaluate the activity of antioxidant medicine with non-invasive real-time measurement.