Automated cell culture in high density tubeless microfluidic device arrays
Automated cell culture in high density tubeless microfluidic device arrays
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DOI：
10.1039/b715375a
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Beebe, David J.
中科院分区：
文献类型：
--
作者：
Meyvantsson, Ivar;Warrick, Jay W.;Beebe, David J.
微流体有望对生命科学研究产生影响。然而，当前的微流体方法与现有的实验室液体分配和检测基础设施不兼容。这种不兼容性是微流体系统采用的障碍，需要改进方法来提高性能并促进生命科学中微流体系统的接受。易用性、标准化接口和自动化仍然是关键挑战。我们提出了一个基于表面张力效应的平台，其中不等体积滴内的压力差驱动被动结构中的流动。我们展示了与现有实验室基础设施的集成、微流体操作（例如无离散微组件的泵送、路由和分隔）以及单层和三维细胞培养中的细胞图案。
Microfluidics is poised to have an impact on life sciences research. However, current microfluidic methods are not compatible with existing laboratory liquid dispensing and detection infrastructure. This incompatibility is a barrier to adoption of microfluidic systems and calls for improved approaches that will enhance performance and promote acceptance of microfluidic systems in the life sciences. Ease of use, standardized interfaces and automation remain critical challenges. We present a platform based on surface tension effects, where the difference in pressure inside drops of unequal volume drives flow in passive structures. We show integration with existing laboratory infrastructure, microfluidic operations such as pumping, routing and compartmentalization without discrete micro-components as well as cell patterning in both monolayer and three-dimensional cell culture.