RESPONSE OF PREOSTEOBLAST AND STEM CELL OF RAT BONE MARROW TO A LETHAL DOSE OF X-IRRADIATION OR CYCLOPHOSPHAMIDE
RESPONSE OF PREOSTEOBLAST AND STEM CELL OF RAT BONE MARROW TO A LETHAL DOSE OF X-IRRADIATION OR CYCLOPHOSPHAMIDE
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DOI：
10.1111/j.1365-2184.1971.tb01536.x
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发表时间：
1971-01-01
期刊：
影响因子：
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通讯作者：
DELL, ES
中科院分区：
文献类型：
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作者：
AMSEL, S;DELL, ES
同种或自体造血组织移植到异位位置后，已观察到在移植组织中发生骨形成。存在于具有成骨潜能的造血组织中的细胞（成骨前细胞）的特性尚不清楚。为了确定该细胞的一些特性，我们将其对X射线照射和环磷酰胺（CTX）的反应与造血干细胞的反应进行了比较。成年雄性大鼠接受900 R全身X射线照射或220 mg/kg腹腔内CTX照射。这两种治疗的剂量都足以使小鼠因骨髓衰竭而死亡。在X射线照射或CTX后的间隔时间内，对造血组织进行检查，以确定是否存在活的造血干细胞和成骨前细胞。X射线照射后，无法检测到活的造血干细胞和成骨前细胞。CTX后可以检测到这些细胞。研究表明，在大鼠中，220 mg/kg的CTX虽然会导致骨髓衰竭死亡，但不会像900 R X‐照射那样有效地减少成骨前细胞或造血干细胞的数量。
Following syngeneic or autotransplantation of hemopoietic tissue to a heterotopic location, bone formation has been observed to occur in the implanted tissue. the characteristics of the cell residing in hemopoietic tissue with bone forming potential (preosteoblast) are unknown. to define some properties of this cell, its response to X‐irradiation and cyclophosphamide (CTX) was compared to the response of the hemopoietic stem cell. Adult, male rats were exposed to 900 R whole body X‐irradiation or 220 mg/kg of intraperitoneal CTX. With either treatment the dose was sufficient to kill the animals by bone marrow failure. At intervals following the X‐irradiation or CTX, hemopoietic tissue was examined for the presence of viable hemopoietic stem cells and preosteoblasts. Following X‐irradiation, viable hemopoietic stem cells and preosteoblasts could not be detected. Following CTX these cells could be detected. It is suggested that in the rat CTX at 220 mg/kg, although causing death by bone marrow failure, does not reduce the population of the preosteoblast or hemopoietic stem cell as effectively as 900 R X‐irradiation.