On-line size-exclusion chromatography/mass spectrometry of low molecular mass heparin
On-line size-exclusion chromatography/mass spectrometry of low molecular mass heparin
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DOI：
10.1002/jms.723
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发表时间：
2004-11-01
影响因子：
2.3
通讯作者：
Roepstorrf, P
中科院分区：
文献类型：
--
作者：
Henriksen, J;Ringborg, LH;Roepstorrf, P
肝素和低分子量肝素（LMMH）由难以分析的硫酸化线性寡糖的复杂混合物组成。一个在线的尺寸排阻色谱/电喷雾离子化（ESI）质谱法，可以确定60多个组件中的LMMH制备。实验装置包括在线阳离子交换，以防止大量的加合在ESI接口。版权所有（C）2004约翰威利父子有限公司。
Heparin and low molecular mass heparin (LMMH) consists of complex mixtures of sulphated linear oligosaccharides that are difficult to analyse. An on-line size exclusion chromatographic/electrospray ionization (ESI) mass spectrometric method that allows the determination of more than 60 components in an LMMH preparation is presented. The experimental setup includes on-line cation exchange in order to prevent massive adducting in the ESI interface. Copyright (C) 2004 John Wiley Sons, Ltd.