Highly sensitive electrochemical immunoassay for zearalenone in grain and grain-based food
Highly sensitive electrochemical immunoassay for zearalenone in grain and grain-based food
复制标题
DOI：
10.1007/s00604-012-0915-1
复制
发表时间：
2013-02
影响因子：
5.7
通讯作者：
Yuankai Wang;Yaxian Yan;Z. Mao;Hengan Wang;Q. Zou;Qianwen Hao;Wenhui Ji;Jianhe Sun
中科院分区：
文献类型：
--
作者：
Yuankai Wang;Yaxian Yan;Z. Mao;Hengan Wang;Q. Zou;Qianwen Hao;Wenhui Ji;Jianhe Sun
We have developed a highly sensitive electrochemical immunoassay for the quantitation of zearalenone (ZEN), a mycotoxin produced byFusariumspecies. In this enzyme linked immunosorbent assay, the enzymatic conversion of the substratep-nitrophenylphosphate is detected by a microplate reader and the signal subsequently converted into an electrical signal. The concentrations of coating antigen (ZEN-ovalbumin), of monoclonal antibody, and of goat anti-mouse antibody labeled with alkaline phosphatase were optimized. In terms of electrochemical detection, the types and pH values of the buffers, the conditions for agitating, and scanning frequency were optimized. The effective detection range of this immunoassay is quite wide (0.004 to 9.5 ng mL−1), and the limit of detection is 2 pg mL−1. ZEN-free corn, wheat, and grain-based food samples were spiked with ZEN and analyzed by this method, and recoveries were found to range from 91.6 % to 113.0 %. Unlike previously described electrochemical methods, this method is both highly sensitive and has a wide working range. The method is fast and thus provides a platform for high-throughput analysis that meets the current need to monitor trace levels of analytes in grain and grain-based food.FigureScheme of test procedure of electrochemical immunosensor (procedure of immune-reaction: from a to f)
