Life Cycle Analysis of Endosymbiotic Algae in an Endosymbiotic Situation with Paramecium bursaria Using Capillary Flow Cytometry
Life Cycle Analysis of Endosymbiotic Algae in an Endosymbiotic Situation with Paramecium bursaria Using Capillary Flow Cytometry
复制标题
使用毛细管流式细胞仪分析草履虫内共生情况下的内共生藻类的生命周期
DOI：
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Toshiyuki Takahashi
中科院分区：
文献类型：
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作者：
Toshiyuki Takahashi
沿着藻类在生态系统和工业应用中作为生产者，一些微藻通过与其他生物的内共生而存在于特殊的生态位中，代表了生物进化的迷人例子。虽然在许多情况下很难通过实验再现内共生，但几种正在进行的内共生是可能的。绿草履虫的内共生是一个特别优秀的模型。虽然许多研究的P. bursaria专门检查感染过程，如主机识别的共生体，协调主机共生体分裂，这已被探索的真核细胞器，是值得追求的。评估藻类的细胞（生命）周期对于藻类应用至关重要。流式细胞术（FCM）已被用于研究包括微藻在内的几种真核细胞的细胞周期。然而，显微镜主要用于研究内共生，如P. bursaria，因为它们的尺寸比适合FCM的细胞更大。大量的时间被花费在微观分析上。本文介绍了一种利用毛细管流式细胞术研究玉蜀黍内共生现象的方法。结果表明，内共生的P. bursaria藻类微调其细胞周期的时间表与他们舒适的主机，并表明一个一致的内共生体-主机的生活周期几乎是确保在他们的内共生。
Along with algae as producers in ecosystems and industrial applications, some microalgae existing in special ecological niches through endosymbiosis with other organisms represent fascinating examples of biological evolution. Although reproducing endosymbiosis experimentally is difficult in many situations, endosymbiosis of several ongoing types is possible. Endosymbiosis in Paramecium bursaria is a particularly excellent model. Although many studies of P. bursaria have specifically examined infection processes such as the host recognition of symbionts, coordination of host-symbiont division, which has been explored for eukaryotic organelles, is worth pursuing. Evaluating the cell (life) cycle of algae is crucially important for algal applications. Flow cytometry (FCM) has been used to study cell cycles of several eukaryotic cells including microalgae. Microscopy, however, has been used mainly to study endosymbiosis, as with P. bursaria, because of their larger size than suitable cells for FCM with hydrodynamic focusing. Vast amounts of time have been expended for microscopic analysis. This review presents an approach using capillary FCM to elucidate the endosymbiosis of P. bursaria. Results reveal that endosymbiotic algae of P. bursaria finely adjust their cell cycle schedule with their comfortable host and show that a coincident endosymbiont–host life cycle is virtually assured in their endosymbiosis.
影响因子：
4.6
作者：
Bucher M;Wolfowicz I;Voss PA;Hambleton EA;Guse A
通讯作者：
Guse A
影响因子：
3.3
作者：
Rauch C;Vries Jd;Rommel S;Rose LE;Woehle C;Christa G;Laetz EM;Wägele H;Tielens AG;Nickelsen J;Schumann T;Jahns P;Gould SB
通讯作者：
Gould SB