Pharmaceutical and biomedical applications of affinity chromatography: recent trends and developments.
Pharmaceutical and biomedical applications of affinity chromatography: recent trends and developments.
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DOI：
10.1016/j.jpba.2012.01.004
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发表时间：
2012-10
影响因子：
3.4
通讯作者：
Zheng, Xiwei
中科院分区：
文献类型：
--
作者：
Hage, David S.;Anguizola, Jeanethe A.;Bi, Cong;Li, Rong;Matsuda, Ryan;Papastavros, Efthimia;Pfaunmiller, Erika;Vargas, John;Zheng, Xiwei
关键词：
亲和层析是一种分离技术，在生物样品和药物制剂的工作中变得越来越重要。这种方法的基础是使用与生物相关的试剂作为固定相，选择性地保留分析物或研究生物相互作用。本综述讨论了亲和层析的基本原理，并考察了该方法在生物医学和药物分析中的最新进展。讨论了基于传统亲和载体的技术，但重点介绍了亲和色谱柱作为高效液相色谱系统的一部分或与其他分析方法结合使用的方法。考虑的亲和层析的一般形式包括分级洗脱方案、弱亲和层析、亲和提取和亲和耗尽。具体的分离技术包括凝集素亲和层析、硼酸盐亲和层析、免疫亲和层析和固定化金属离子亲和层析。还介绍了用亲和层析研究生物相互作用的方法，如测定平衡常数、速率常数或竞争和置换效应。此外，还简要介绍了固定化酶反应器、分子印迹聚合物、染料配体和核酸适体的应用进展。
Affinity chromatography is a separation technique that has become increasingly important in work with biological samples and pharmaceutical agents. This method is based on the use of a biologically-related agent as a stationary phase to selectively retain analytes or to study biological interactions. This review discusses the basic principles behind affinity chromatography and examines recent developments that have occurred in the use of this method for biomedical and pharmaceutical analysis. Techniques based on traditional affinity supports are discussed, but an emphasis is placed on methods in which affinity columns are used as part of HPLC systems or in combination with other analytical methods. General formats for affinity chromatography that are considered include step elution schemes, weak affinity chromatography, affinity extraction and affinity depletion. Specific separation techniques that are examined include lectin affinity chromatography, boronate affinity chromatography, immunoaffinity chromatography, and immobilized metal ion affinity chromatography. Approaches for the study of biological interactions by affinity chromatography are also presented, such as the measurement of equilibrium constants, rate constants, or competition and displacement effects. In addition, related developments in the use of immobilized enzyme reactors, molecularly imprinted polymers, dye ligands and aptamers are briefly considered.
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影响因子：
4.1
作者：
Bourne, Y;Astoul, CH;Rougé, P
通讯作者：
Rougé, P
影响因子：
2.9
作者：
Chen S;Sobansky MR;Hage DS
通讯作者：
Hage DS
影响因子：
4.3
作者：
Cheshev, Pavel;Morelli, Laura;Bernardi, Anna
通讯作者：
Bernardi, Anna
DOI：
10.1016/j.jchromb.2004.06.012
发表时间：
2004-09-25
影响因子：
3
作者：
Chen, JZ;Ohnmacht, C;Hage, DS
通讯作者：
Hage, DS
影响因子：
2.9
作者：
BOWERSKOMRO, DM;YAMADA, Y;MCCORMICK, DB
通讯作者：
MCCORMICK, DB