Effect of nitric oxide on auditory cortical neurons of aged rats
Effect of nitric oxide on auditory cortical neurons of aged rats
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DOI：
10.1016/j.neulet.2008.09.074
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发表时间：
2008-12-05
影响因子：
2.5
通讯作者：
Yeo, Seung Geun
中科院分区：
文献类型：
--
作者：
Lee, Jong-Ju;Cho, Young-Wuk;Yeo, Seung Geun
尚未确定一氧化氮（NO）对听觉皮层神经元的影响中与神经元相关的变化。因此，我们评估了这些神经元的解剖学变化和神经生理学特征的大鼠作为年龄的函数。在24月龄（老龄组）和4月龄（对照组）的Sprague-Dawley大鼠中测量甲酚紫染色细胞的数量、NADPH-d阳性神经元细胞体的数量和面积及其光密度。本实验还观察了NO对急性分离的大鼠听皮层神经元K+电流的调制作用。神经胶质细胞和神经元的分布模式，或NADPH-cl阳性神经元胞体的数量和面积没有组间差异。然而，NADPH-d阳性神经元胞体的光密度在老年组中显著大于对照组。此外，大鼠听皮层神经元的电压门控K+电流被NO水平的增加激活。由于K+电流的激活可能抑制神经元的兴奋性，因此与年龄相关的NO产生的增加可以通过抑制神经元的兴奋性来阻碍听觉中枢的功能。0 2008爱思唯尔爱尔兰有限公司版权所有。
Age-related changes in the effects of nitric oxide (NO) on neurons of the auditory cortex have not been determined. We therefore evaluated the anatomical changes and neurophysiological characteristics of these neurons in rats as a function of age. The numbers of cresyl violet stained cells, the numbers and areas of NADPH-d-positive neuronal cell bodies, and their optical density, were measured in Sprague-Dawley rats aged 24 months (aged group) and 4 months (control group). The modulatory effects of NO on K+ currents of acutely isolated rat auditory cortical neurons were also assessed. There were no between-group differences in the distribution patterns of glial cells and neurons, or in the numbers and areas of NADPH-cl-positive neuronal cell bodies. However, the optical density of NADPH-d-positive neuronal cell bodies was significantly greater in the aged group than in the control group. in addition, voltage-gated K+ currents of rat auditory cortical neurons were activated by increased levels of NO. As activation of the K+ current likely suppresses neuronal excitability, age-associated increases in NO production can hinder the function of the acoustic center by inhibiting neuron excitability. 0 2008 Elsevier Ireland Ltd. All rights reserved.