A DNA barcode for Piroplasmea
A DNA barcode for Piroplasmea
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Piroplasmea 的 DNA 条形码
DOI：
10.1016/j.actatropica.2012.07.001
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发表时间：
2012-10-01
期刊：
影响因子：
2.7
通讯作者：
Luo, Jianxun
中科院分区：
文献类型：
--
作者：
Gou, Huitian;Guan, Guiquan;Luo, Jianxun
由于形态学鉴定的困难，发展可靠的分子工具来区分物种是梨浆虫的优先事项。先前基于18 S rRNA和其他基因序列的研究为梨浆虫的生殖发育提供了基础。然而，在一个全面的样本中很难区分物种。在这里，8个DNA区域，包括18 S rRNA，285 rRNA，内转录间隔区（ITS）区域和COI基因的能力，已经比较了作为候选人的DNA条形码的梨浆虫。从本研究和GenBank中共收集到484条梨浆虫基因序列。根据生命条形码联盟（CBOL）的标准评估了8个拟议的DNA区域。从这次评估中。ITS 2的PCR扩增效率为100%，序列长度理想，种内和种间差异最大，属级鉴定效率为98%，种级鉴定效率为92%。因此，我们建议ITS 2是最理想的DNA条形码的基础上，目前的数据库piroplasma。(C)2012爱思唯尔有限公司版权所有。
Due to the difficulty in morphological identification the development of reliable molecular tools for species distinction is a priority for piroplasma. Previous studies based on 18S rRNA and other gene sequences provided a backbone for the phylogeny of piroplasma. However, it is difficult to discriminate species in a comprehensive sample. Here, the abilities of eight DNA regions including 18S rRNA, 285 rRNA, internal transcribed spacer (ITS) regions and COI genes, have been compared as candidates of DNA barcodes for piroplasma. In total, 484 sequences of piroplasma were collected from this study and GenBank. The eight proposed DNA regions were evaluated according to the criterion of Consortium for the Barcode of Life (CBOL). From this evaluation. ITS2 had 100% PCR amplification efficiency, an ideal sequence length, the largest gap between the intra- and inter-specific divergence, 98% identification efficiency at the genus level, and 92% at the species level. Thus, we propose that ITS2 is the most ideal DNA barcode based on the current database for piroplasma. (C) 2012 Elsevier B.V. All rights reserved.