Maternal transmission of mutans Streptococci in severe-early childhood caries.
Maternal transmission of mutans Streptococci in severe-early childhood caries.
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发表时间：
2009-05
影响因子：
1.6
通讯作者：
Stephen C. Mitchell;J. Ruby;S. Moser;Stephanie S Momeni;Anita Smith;R. Osgood;M. Litaker;N. Childers
中科院分区：
文献类型：
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作者：
Stephen C. Mitchell;J. Ruby;S. Moser;Stephanie S Momeni;Anita Smith;R. Osgood;M. Litaker;N. Childers
本研究利用多种遗传学分析来检测患有S-ECC的儿童中母亲获得MS的证据。方法27对5-ECC患儿在全麻下行牙体修复前的母亲/儿童对。从母亲、孩子和孩子的龋损处收集牙菌斑样本。引物聚合酶链反应（AP-PCR）对每个斑块样本中的6-8个MS分离株进行基因分型，并鉴定出独特的基因型。具有独特基因型的代表性MS分离株的特征在于脉冲场凝胶电泳（PFGE）。使用基于Dice条带的相似性系数进行聚类分析，根据凝胶键合模式生成树状图。Dice系数>70%表明PFGE基因型间存在相似性或匹配性。结果在26%（7/27）的母亲/孩子对中，所有孩子的分离株都与母亲匹配。在15%（4/27）中，一些孩子的基因型与母亲匹配，在59%（16/27）中，没有分离物与母亲匹配。41%（11/27）的母亲/儿童MS获得模式为母体传播，而74%（20/27）的母亲/儿童MS获得模式为非母体传播。结论：在该S-ECC人群中，在大多数儿童（74%）中发现了与母体菌株不匹配的MS基因型。在41%的母婴配对中发现了母婴传播的证据。
PURPOSE This study utilized multiple genetic analyses to detect evidence of maternal MS acquisition in children with S-ECC. METHODS Twenty-seven mother/child pairs were selected from children with 5-ECC preceding dental rehabilitation under general anesthesia. Plaque samples were collected from the mother, child, and the child's carious lesion. Arbitrarily primed-polymerase chain reaction (AP-PCR) genotyped 6-8 MS isolates from each plaque sample, and unique genotypes were identified. Representative MS isolates with unique genotypes were characterized by pulsed-field gel electrophoresis (PFGE). Cluster analysis using the Dice band-based similarity coefficient was used to generate dendrograms from gel bonding patterns. A Dice coefficient >70% indicated similarity or match among PFGE genotypes. RESULTS In 26% (7/27) of mother/child pairs, all of the child's isolates matched the mother. In 15% (4/27), some of the child's genotypes matched the mother, and in 59% (16/27), no isolates matched the mother. Maternal transmission was a mode of MS acquisition in 41% (11/27) of mother/child pairs, while acquisition from non-maternal sources occurred in 74% (20/27). CONCLUSIONS MS genotypes that did not match maternal strains were identified in the majority of children (74%) within this S-ECC population. Evidence of maternal transmission was detected in 41% of mother/child pairs.