Surface-sampling mass spectrometry to study proteins and protein complexes.
Surface-sampling mass spectrometry to study proteins and protein complexes.
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DOI：
10.1042/ebc20220191
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发表时间：
2023-03-29
影响因子：
6.4
通讯作者：
中科院分区：
文献类型：
--
作者：
本文旨在总结目前提供完整蛋白质分析的表面质谱学(MS)方法和非共价复合体的能力。蛋白质分析在很大程度上是通过基质辅助激光解吸/电离(MALDI)实现的，这本身就是一种表面分析方法或基于溶剂的电喷雾电离(ESI)。基于ESI已经开发了几种表面采样方法，下面将讨论用于完整蛋白质分析的那些方法。对于每种方法，将讨论蛋白质覆盖的范围、自上而下的阐明以及对天然蛋白质和非共价复合体的蛋白质结构的探测。还将讨论改进蛋白质分析的战略，从样品制备和采样方法到仪器改进以及将离子迁移率分离纳入工作流程。将总结每种方法的相对优点和缺点，提供对当前能力的概述。
This review aims to summarise the current capabilities of surface mass spectrometry (MS) approaches that offer intact protein analysis, and that of non-covalent complexes. Protein analysis is largely achieved via matrix-assisted laser desorption/ionisation (MALDI), which is in itself a surface analysis approach or solvent-based electrospray ionisation (ESI). Several surface sampling approaches have been developed based on ESI, and those that have been used for intact protein analysis will be discussed below. The extent of protein coverage, top-down elucidation, and probing of protein structure for native proteins and non-covalent complexes will be discussed for each approach. Strategies for improving protein analysis, ranging from sample preparation, and sampling methods to instrument modifications and the inclusion of ion mobility separation in the workflow will also be discussed. The relative benefits and drawbacks of each approach will be summarised, providing an overview of current capabilities.
DOI：
10.1002/rcm.8246
发表时间：
2021-01
期刊：
Rapid communications in mass spectrometry : RCM
影响因子：
--
作者：
Hale OJ;Ryumin P;Brown JM;Morris M;Cramer R
通讯作者：
Cramer R
DOI：
10.1007/s13361-016-1511-0
发表时间：
2016-12
影响因子：
3.2
作者：
Ait-Belkacem, Rima;Dilillo, Marialaura;Pellegrini, Davide;Yadav, Avinash;de Graaf, Erik L.;McDonnell, Liam A.
通讯作者：
McDonnell, Liam A.
影响因子：
7.4
作者：
Edwards, Rebecca L.;Creese, Andrew J.;Baumert, Mark;Griffiths, Paul;Bunch, Josephine;Cooper, Helen J.
通讯作者：
Cooper, Helen J.