Characterization of diarrhetic shellfish toxins in the Mizuhopecten yessoensis (scallop) midgut gland by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
Characterization of diarrhetic shellfish toxins in the Mizuhopecten yessoensis (scallop) midgut gland by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
复制标题
采用高效液相色谱-串联质谱 (HPLC-MS/MS) 表征虾夷扇贝中肠腺中的腹泻性贝类毒素
DOI：
10.1080/00032719.2022.2092633
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发表时间：
2023
影响因子：
2
通讯作者：
Shinsuke Inagaki
中科院分区：
文献类型：
--
作者：
Takashi Yarita;Shinsuke Inagaki
液相色谱-串联质谱法（LC-MS/MS）作为日本官方方法的一部分，常规应用于腹泻性贝类中毒（DSP）相关毒素的测定。对于扇贝，不仅可食用部分，而且中肠腺也可用于分析。本研究采用LC-MS/ ms法测定扇贝中肠腺中冈田酸（OA）和甲藻毒素-1 (DTX1)，评价基质效应对测定结果准确性的影响。在实验室间比较研究中分析的样品采用日本官方方法。通过和不通过清洗过程（使用C18滤筒的正己烷洗涤和固相萃取的组合）得到的溶液被稀释到不同的比例并用于评估。对于所有制备的溶液，与甲醇中的样品相比，OA和DTX1的电离增强；清理过程并没有充分降低基质效应。虽然样品溶液的稀释降低了基质效应，但改善是有限的。另一种预处理技术是准确测定该基质中dsp相关毒素所必需的。
Liquid chromatography – tandem mass spectrometry (LC-MS/MS) is routinely applied for the determination of toxins associated with diarrhetic shellfish poisoning (DSP) as a part of the Japanese official method. For scallops, not only the edible part but also the midgut gland may be used for analysis. In this study, the matrix effects in the LC-MS/MS-based determination of okadaic acid (OA) and dinophysistoxin-1 (DTX1) in the scallop midgut gland were evaluated to assess the impact on the accuracy of the results. The analyzed sample in an interlaboratory comparison study was subjected to the Japanese official method. The solutions obtained with and without a cleanup process (combination of hexane washing and solid-phase extraction using a C18 cartridge) were diluted to varying ratios and used for evaluation. For all prepared solutions, the ionization of OA and DTX1 was enhanced compared to the samples in methanol; the cleanup process did not sufficiently reduce the matrix effects. Although the dilution of the sample solution reduced the matrix effect, the improvement was limited. Another pretreatment technique is necessary for the accurate determination of DSP-associated toxins in this matrix.