Complement receptor 1/CD35 is a receptor for mannan-binding lectin.
Complement receptor 1/CD35 is a receptor for mannan-binding lectin.
复制标题
补体受体1/CD35是曼南结合凝集素的受体。
DOI：
10.1084/jem.192.12.1797
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发表时间：
2000-12-18
期刊：
影响因子：
--
通讯作者：
Nicholson-Weller A
中科院分区：
文献类型：
--
作者：
Ghiran I;Barbashov SF;Klickstein LB;Tas SW;Jensenius JC;Nicholson-Weller A
甘露聚糖结合凝集素(MBL)是凝集素家族中的一员，具有调理功能，但其细胞受体的鉴定一直难以确定。与MBL同源的补体C1q与补体受体1(CR1/CD35)结合，因此我们研究了CR1是否也具有MBL受体的功能。放射性碘标记的MBL与固定在塑料上的重组可溶性CR1(SCR1)结合，其表观平衡解离常数为5 nM。N-乙酰-d-氨基葡萄糖不抑制sCR1-MBL的结合，表明MBL的糖结合部位不参与CR1的结合。C1q抑制MBL与固定化sCR1的结合，提示MBL和C1q可能与CR1上相同或相邻的位点结合。与中性粒细胞(PMN)结合的MBL与佛波醇肉豆蔻酸酯诱导的CR1表达变化呈正相关。最后，CR1介导了人红细胞与固定化MBL的黏附，并作为MBL免疫球蛋白G调理细菌的PMN的吞噬受体发挥作用。因此，MBL同时与重组的sCR1和细胞CR1结合，从而支持CR1作为集合素MBL的细胞受体的作用。
Mannan-binding lectin (MBL), a member of the collectin family, is known to have opsonic function, although identification of its cellular receptor has been elusive. Complement C1q, which is homologous to MBL, binds to complement receptor 1 (CR1/CD35), and thus we investigated whether CR1 also functions as the MBL receptor. Radioiodinated MBL bound to recombinant soluble CR1 (sCR1) that had been immobilized on plastic with an apparent equilibrium dissociation constant of 5 nM. N-acetyl-d-glucosamine did not inhibit sCR1–MBL binding, indicating that the carbohydrate binding site of MBL is not involved in binding CR1. C1q inhibited MBL binding to immobilized sCR1, suggesting that MBL and C1q might bind to the same or adjacent sites on CR1. MBL binding to polymorphonuclear leukocytes (PMNs) was associated positively with changes in CR1 expression induced by phorbol myristate acetate. Finally, CR1 mediated the adhesion of human erythrocytes to immobilized MBL and functioned as a phagocytic receptor on PMNs for MBL–immunoglobulin G opsonized bacteria. Thus, MBL binds to both recombinant sCR1 and cellular CR1, which supports the role of CR1 as a cellular receptor for the collectin MBL.
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影响因子：
2.7
作者：
KAWASAKI, N;KAWASAKI, T;YAMASHINA, I
通讯作者：
YAMASHINA, I
DOI：
10.1073/pnas.96.19.10794
发表时间：
1999-09-14
影响因子：
11.1
作者：
Holmskov, U;Mollenhauer, J;Skjodt, K
通讯作者：
Skjodt, K
影响因子：
20.3
作者：
Kumar, A;Wetzler, E;Berger, M
通讯作者：
Berger, M
DOI：
10.1084/jem.168.5.1699
发表时间：
1988-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Klickstein LB;Bartow TJ;Miletic V;Rabson LD;Smith JA;Fearon DT
通讯作者：
Fearon DT
影响因子：
32.4
作者：
Klickstein, LB;Barbashov, SF;NicholsonWeller, A
通讯作者：
NicholsonWeller, A