Unravelling diversity of deep-sea copepods using integrated morphological and molecular techniques
Unravelling diversity of deep-sea copepods using integrated morphological and molecular techniques
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DOI：
10.1093/plankt/fbx031
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发表时间：
2017-07-01
影响因子：
2.1
通讯作者：
Cornils, Astrid
中科院分区：
文献类型：
--
作者：
Bode, Maya;Laakmann, Silke;Cornils, Astrid
准确的物种鉴定是生态学研究的关键。对于桡足类，这通常是基于一些诊断的形态特征，这可能是高度保守的，导致低估了许多桡足类家庭的物种多样性。我们阐明物种丰富的形态学上具有挑战性和生态重要性的深海桡足类家庭Spinocalanidae在热带大西洋应用综合分类方法相结合的形态，DNA序列分析和蛋白质指纹。总共有28个形态种可以区分，而39个推定的物种被检测到使用DNA序列分析和42个蛋白质组指纹图谱。这一结果验证了蛋白质组指纹，以简化和加速未来的生物多样性研究桡足类高分类分辨率。我们的研究结果表明，这种综合形态和分子分类方法的力量，揭示了大量的神秘或pseudocryptic物种，从而揭示了不完整的分类指南，这组在探索不力的中层境界。此外，我们的分析揭示了Mospicalanus和Spinocalanus A组的密切关系，并表明Spinocalanus属可能是复系的。被低估的物种多样性表明，在广大但研究不足的中层水域中，捕食者-被捕食者关系、种间竞争和物种特异性专业化方面存在复杂的生态相互作用。
Accurate species identification is crucial for ecological studies. For copepods, this is usually based on a few diagnostic morphological characters, which can be highly conserved, resulting in an underestimation of species diversity in many copepod families. We elucidate species richness in the morphologically challenging and ecologically important deep-sea copepod family Spinocalanidae in the tropical Atlantic by applying an integrated taxonomic approach combining morphology, DNA-sequence analyses and proteomic fingerprinting. In total, 28 morphospecies could be discriminated, while 39 putative species were detected using DNA-sequence analyses and 42 using proteomic fingerprinting. This outcome verifies proteomic fingerprinting to simplify and accelerate future biodiversity studies of copepods with high taxonomic resolution. Our findings demonstrate the power of this integrated morphological and molecular taxonomic approach by revealing high numbers of cryptic or pseudocryptic species and thus uncovering the incompleteness of taxonomic guides for this group in the poorly explored mesopelagic realm. Furthermore, our analyses reveal a close relationship of Mospicalanus and Spinocalanus group A and indicate that the genus Spinocalanus may be polyphyletic. The underestimated species diversity suggests complex ecological interactions in terms of predator-prey relationships, interspecific competition and species-specific specializations in the vast, but under-studied mesopelagic realm.