A dually located multi-HMG-box protein of Aspergillus nidulans has a crucial role in conidial and ascospore germination
A dually located multi-HMG-box protein of Aspergillus nidulans has a crucial role in conidial and ascospore germination
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DOI：
10.1111/mmi.12772
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发表时间：
2014-10-01
影响因子：
3.6
通讯作者：
Hamari, Zsuzsanna
中科院分区：
文献类型：
--
作者：
Karacsony, Zoltan;Gacser, Attila;Hamari, Zsuzsanna
已在基因组数据库中鉴定出7个Nidulans曲霉HMG-box蛋白。其中三个具有非特异DNA结合蛋白的特征。其中之一，AN1267(HMBB)，在其C-末端包括一个典型的HMG-盒，并且在正则盒的上游有两个结构相关的盒，被称为影子-HMG-盒。该蛋白与鹅座孢霉mtHMG1一起，定义了一类存在于拟青霉中的蛋白质，以及一些丝状真菌中的同源蛋白。HmbB主要定位于线粒体，但偶尔也定位于细胞核。同源基因的缺失导致了一系列的多效性效应，包括对菌丝形态的影响，对氧化胁迫的敏感性，杂色囊藻毒素的缺乏和分生孢子代谢产物的变化。缺失菌株最显著的表型是分生孢子和子囊孢子活力急剧下降。我们表明，这很可能是因为这种蛋白质对维持孢子中的线粒体DNA是必不可少的。
Seven HMG-box proteins of Aspergillus nidulans have been identified in the genomic databases. Three of these have the characteristics of non-specific DNA-binding proteins. One of these, AN1267 (HmbB), comprises one canonical HMG-box in its C-terminus and upstream of the canonical box two structurally related boxes, to be called Shadow-HMG-boxes. This protein defines, together with the Podospora anserina mtHMG1, a clade of proteins present in the Pezizomycotina, with orthologues in some of the Taphrinomycotina. HmbB localizes primarily to the mitochondria but occasionally in nuclei. The deletion of the cognate gene results in a number of pleiotropic effects, including those on hyphal morphology, sensitivity to oxidative stress, absence of sterigmatocystin production and changes in the profile of conidial metabolites. The most striking phenotype of deletion strains is a dramatic decrease in conidial and ascospore viability. We show that this is most likely due to the protein being essential to maintain mitochondrial DNA in spores.