In vitro splicing of pre-messenger RNA with extracts from 5-fluorouridine-treated cells.
In vitro splicing of pre-messenger RNA with extracts from 5-fluorouridine-treated cells.
复制标题
前信使 RNA 与 5-氟尿苷处理细胞提取物的体外剪接。
DOI：
10.1042/bj2970297
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
J. R. Patton
中科院分区：
文献类型：
--
作者：
J. R. Patton
The effect of 5-fluorouridine (5-FU) treatment of cells on the splicing of pre-mRNA was determined using cellular extracts and splicing in vitro. Nuclear extracts from control cells and cells treated with 5-FU were prepared and used to splice pre-mRNAs in vitro. The drug treatment resulted in inhibition of cell growth but had little effect on RNA synthesis. The extracts from 5-FU-treated cells showed significant inhibition of splicing. This inhibition was the result of reduced efficiency and was not caused by a block at a specific step in the splicing pathway. There were no observable changes in the levels or physical properties of the small nuclear ribonucleoprotein particles that are essential cofactors in the splicing process. The deficiency in splicing in the extracts from 5-FU-treated cells could be supplemented by the addition of complementary fractions from a control extract.
