Confocal microscopic observation of structural changes in glass-ionomer cements and tooth interfaces.
Confocal microscopic observation of structural changes in glass-ionomer cements and tooth interfaces.
复制标题
玻璃离子水门汀和牙齿界面结构变化的共焦显微镜观察。
DOI：
10.1016/s0142-9612(97)00140-3
复制
发表时间：
1998
期刊：
影响因子：
14
通讯作者：
M. Naasan
中科院分区：
文献类型：
--
作者：
T. Watson;D. Pagliari;S. Sidhu;M. Naasan
本研究旨在开发能够在放置玻璃离聚物修复材料之前、期间和之后对空腔进行动态成像的技术。在最近拔除的第三磨牙中切出蛀牙，并将牙齿纵向切片。将每个半切的牙齿表面放置在与显微镜光轴成 90° 的绿色模型化合物中。使用视频速率共焦显微镜结合内部可聚焦显微镜物镜对腔表面进行成像。载物台上的样品被推到物镜处，物镜将盖玻片“夹”在其上。将水、甘油或油置于盖玻片下方，油置于上方。通过改变物镜的内焦点来对内牙结构进行成像。然后将修复材料放入空腔中。视频图像使用图像采集卡、计算机和大容量存储器以数字方式存储在录像带上。软件控制随着时间的推移产生界面的延时记录。初步实验检查了传统玻璃离子水门汀和可注射树脂改性玻璃离子水门汀的放置和早期成熟。在形成粘合之前，当塑料材料滚过牙釉质和牙本质时，可以看到水泥基体和玻璃颗粒的初始接触。还发现了水从牙本质移动到水泥中的证据。固化后，使用延时设备可以明显看出由于水通量而引起的水泥的早期尺寸变化。这项新技术能够检查正在发育的牙齿/修复体界面并跟踪材料的运动。
This study aimed to develop techniques to allow dynamic imaging of a cavity before, during and after placement of glass-ionomer restorative materials. Cavities were cut in recently extracted third molars and the teeth longitudinally sectioned. Each hemisected tooth surface was placed in green modelling compound at 90° to the optical axis of the microscope. The cavity surface was imaged using a video rate confocal microscope in conjunction with an internally focusable microscope objective. The sample on the stage was pushed up to the objective lens which ‘clamped’ the cover glass onto it. Water, glycerine or oil was placed below the coverglass, with oil above. Internal tooth structures were imaged by changing the internal focus of the objective. The restorative material was then placed into the cavity. Video images were stored either onto video tape or digitally, using a frame grabber, computer and mass memory storage. Software controls produced time-lapse recordings of the interface over time. Preliminary experiments have examined the placement and early maturation of conventional glass-ionomer cements and a syringeable resin-modified glass-ionomer cement. Initial contact of the cement matrix and glass particles was visible as the plastic material rolled past the enamel and dentine, before making a bond. Evidence for water movement from the dentine into the cement has also been seen. After curing, the early dimensional changes in the cements due to water flux were apparent using the time-lapse facility. This new technique enables examination of developing tooth/restoration interfaces and the tracking of movement in materials.