Immunocytochemical studies of cathepsin D in human skeletal muscle
Immunocytochemical studies of cathepsin D in human skeletal muscle
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人骨骼肌组织蛋白酶 D 的免疫细胞化学研究
DOI：
10.1002/ana.410130205
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发表时间：
1983
影响因子：
11.2
通讯作者：
J. Mendell
中科院分区：
文献类型：
--
作者：
J. Whitaker;T. Bertorini;J. Mendell
组织蛋白酶 D（一种酸性内肽酶）的分布通过免疫细胞化学定位在从 34 名患有各种神经肌肉疾病的人身上获得的人骨骼肌中。正常人类骨骼肌含有少量组织蛋白酶 D，所有这些都被发现靠近肌膜。免疫反应性组织蛋白酶 D 存在于许多浸润吞噬细胞的细胞质中，并且在肌营养不良、炎性肌病、横纹肌溶解、酸性麦芽糖酶缺乏和神经源性萎缩患者的骨骼肌纤维中增加。在杜兴型肌营养不良症的病例中，组织蛋白酶 D 的增加在小再生纤维中尤其明显，在溶酶体外位置的溶酶体样细胞器的超微结构水平上可见。组织蛋白酶 D 在骨骼肌中的功能尚不清楚，但目前的研究结果表明其可能在肌肉再生和修复中发挥作用。如果要将干扰蛋白水解活性的药物用作治疗神经肌肉疾病的治疗剂，则必须仔细选择干扰蛋白水解活性的药物。
The distribution of cathepsin D, an acidic endopeptidase, was localized by immunocytochemistry in human skeletal muscle obtained from 34 persons with a variety of neuromuscular disorders. Normal human skeletal muscle contained small amounts of cathepsin D, all of which was found close to the sarcolemmal membrane. Immunoreative cathepsin D was present in the cytoplasm of many infiltrating phagocytic cells and was increased in skeletal muscle fibers from patients with muscular dystrophies, inflammatory myopathies, rhabdomyolysis, acid maltase deficiency, and neurogenic atrophy. In cases of Duchenne type muscular dystrophy, the increase in cathepsin D was especially prominent in small regenerating fibers, in which it was visualized at the ultrastructural level in lysosome‐like organelles anextralysosomal locations. The function of cathepsin D in skeletal muscle is unclear, but the present findings suggest a possible role in muscle regeneration and repair. Such a role would necessitate careful selection of drugs which interfere with proteolytic activity if they are to be used as therapeutic agents in treating neuromuscular diseases.
甲状腺机能亢进兔心脏、骨骼肌和肝脏的溶酶体改变。
DOI：
--
发表时间：
1981
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Decker,RS;Wildenthal,K
通讯作者：
Wildenthal,K