Nanopore Stochastic Detection of a Liquid Explosive Component and Sensitizers Using Boromycin and an Ionic Liquid Supporting Electrolyte
Nanopore Stochastic Detection of a Liquid Explosive Component and Sensitizers Using Boromycin and an Ionic Liquid Supporting Electrolyte
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DOI：
10.1021/ac801877g
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发表时间：
2009-01-01
影响因子：
7.4
通讯作者：
Guan, Xiyun
中科院分区：
文献类型：
--
作者：
Jayawardhana, Dilani A.;Crank, Jeffrey A.;Guan, Xiyun
我们报告了一种快速、灵敏的随机纳米孔传感方法，用于检测单价阳离子和液体爆炸成分及其敏化剂。传感元件是野生型α-溶血素蛋白孔，以硼霉素作为分子接头，而含有离子液体的溶液作为背景电解质。分析物-硼霉素复合物显示出显着不同的特征。具体来说，它们的事件平均停留时间和幅度足够不同，可以方便地区分甚至同时检测水环境中的液体爆炸物成分。此外，结果还表明，使用特定的离子液体盐溶液代替 NaCl 或 KCl 溶液作为支持电解质，提供了一种有效的手段，可以大大提高随机传感中纳米孔对某些分析物的灵敏度。
We report a rapid and sensitive stochastic nanopore sensing method for the detection of monovalent cations and liquid explosive components and their sensitizers. The sensing element is a wild-type alpha-hemolysin protein pore with boromycin as a molecular adaptor, while a solution containing an ionic liquid was used as the background electrolyte. The analyte-boromycin complexes showed significantly different signatures. Specifically, their event mean dwell times and amplitudes were sufficiently distinct to permit the convenient differentiation and even simultaneous detection of liquid explosive components in aqueous environments. In addition, the results also demonstrate that the usage of specific ionic liquid salt solutions instead of NaCl or KCl solution as supporting electrolyte provides a useful means to greatly enhance the sensitivity of the nanopore for some analytes in stochastic sensing.