LC-MS compatible HPLC separation for xenobiotics and their phase I and phase II metabolites: simultaneous anion exchange and reversed-phase chromatography.
LC-MS compatible HPLC separation for xenobiotics and their phase I and phase II metabolites: simultaneous anion exchange and reversed-phase chromatography.
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LC-MS 兼容的 HPLC 分离异生素及其 I 相和 II 相代谢物：同步阴离子交换和反相色谱。
DOI：
10.1093/chromsci/29.12.511
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发表时间：
1991
影响因子：
1.3
通讯作者：
Stephens,RD
中科院分区：
文献类型：
--
作者：
Johnston,JJ;Draper,WM;Stephens,RD
苯及其五种哺乳动物代谢产物苯酚、苯基-β-d-葡萄糖醛酸苷、苯硫酸盐、苯巯基尿酸盐和t，t-粘康酸在甲基苯乙烯-二乙烯基苯阴离子交换反相柱上得到分离。通过改变移动的相缓冲液的类型、离子强度和pH以及有机移动的相改性剂的类型和百分比来操纵模型化合物的保留。开发的分离与液相色谱-质谱（LC-MS）中使用的粒子束（PB）和热喷雾（TSP）接口兼容。这些分离应适用于广泛的异生物质及其I相和II相代谢物的研究，并提供了一个LC-MS兼容的替代使用离子配对试剂。
Benzene and five of its mammalian metabolites, phenol, phenyl-β-d-glucuronide, phenylsulfate, phenylmercapturate, andt,t-muconic acid, are separated on a methylstyrene-divinylbenzene-based anion exchange—reversed-phase column. The retention of the model compounds is manipulated by modifying the type, ionic strength, and pH of the mobile phase buffer, and the type and percent of organic mobile phase modifier. The separations developed are compatible with both particle beam (PB) and thermospray (TSP) interfaces used in liquid chromatography—mass spectrometry (LC-MS). These separations should be adaptable to the study of a wide range of xenobiotics and their phase I and phase II metabolites, and provide an LC—MS compatible alternative to the use of ion pairing reagents.