Molecular mechanism underlying the differential MYF6 expression in postnatal skeletal muscle of Duroc and Pietrain breeds
Molecular mechanism underlying the differential MYF6 expression in postnatal skeletal muscle of Duroc and Pietrain breeds
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DOI：
10.1016/j.gene.2011.06.031
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发表时间：
2011-10-15
期刊：
影响因子：
3.5
通讯作者：
Schellander, Karl
中科院分区：
文献类型：
--
作者：
Fan, Huitao;Cinar, Mehmet Ulas;Schellander, Karl
在现代西方猪中，杜洛克猪（高肉脂比）和皮特兰猪（低肉脂比）在商业猪肉生产中广泛使用的品种在肌肉表型上存在极大差异。这些品种特异性差异的分子机制，特别是表观遗传机制知之甚少。肌源性因子6（Myogenic factor 6，MYF 6）是在成人肌肉中表达最丰富的肌源性因子。此外，MYF 6倾向于在瘦肉型选择系的肌肉组织中更高地表达，并且被认为是一个有希望的成年猪生长和肉质相关性状的候选基因。本研究使用6月龄雌性杜洛克和皮特兰纯种猪。分别采用Western blotting和实时荧光定量PCR（qRT-PCR）方法检测眼肌中MYF 6蛋白和mRNA的表达水平。通过亚硫酸氢盐测序PCR（BSP）确定MYF 6 5 '调节区的DNA甲基化状态。通过蛋白质印迹法检测组蛋白4在赖氨酸5（H4 K5）和8（H4 K8）处的整体乙酰化。皮特兰猪的MYF 6和MYF 6 5 '调控区高甲基化E2 F1结合元件的表达显著高于杜洛克猪。在皮特兰猪中观察到DNA甲基转移酶1（DNMT 1）表达的显著升高，这与MYF 6的超甲基化一致。H4 K5和H4 K8组蛋白乙酰化水平在两个品种猪之间均不显著。然而，E2 F1的mRNA和蛋白质表达显着升高的皮特兰品种。因此，可以想象，出生后皮特兰猪中MYF 6转录的上调与MYF 6 5 '调控区的表观遗传修饰引起的顺式激活无关，但可能归因于E2 F1富集表达引起的反式激活。(C)2011爱思唯尔有限公司版权所有。
Among modern western pigs, Duroc (high meat fat ratio) and Pietrain (low meat fat ratio) breeds extensively utilized in commercial pork production differ extremely for their muscle phenotypes. The molecular mechanism, especially the epigenetic mechanism, underlying these breed-specific differences is poorly known. Myogenic factor 6 (MYF6) is the most abundantly expressed myogenic factor in adult muscle. Moreover, MYF6 tends to be expressed more highly in muscle tissue of the lean selection line and is supposed to be one promising candidate gene for growth- and meat quality-related traits in adult pigs. Six months old female Duroc and Pietrain pure breed pigs were used in this study. Protein and mRNA levels of MYF6 in loin eye muscle were determined by Western blotting and quantitative Real-time reverse transcription PCR (qRT-PCR), respectively. The DNA methylation status of the MYF6 5'-regulatory region was determined by bisulfite sequencing PCR (BSP). The global Histone 4 acetylation at lysines 5 (H4K5) and 8 (H4K8) were examined by Western blotting. Pietrain pigs exhibited significant higher expression of MYF6 and hypermethylated E2F1 binding element within MYF6 5'-regulatory region as compared with Duroc pigs. Significant elevation in DNA methyltransferase 1 (DNMT1) expression was observed in Pietrain pigs which are in agreement with hypermethylation of MYF6. Histone acetylation level at neither H4K5 nor H4K8 is significant between two breed pigs. Nevertheless, mRNA and protein expression of E2F1 were significantly elevated in the Pietrain breed. It is thus conceivable that the upregulation of MYF6 transcription in postnatal Pietrain pigs is not associated with cis-activation by epigenetic modification of MYF6 5'-regulatory region, but may be attributed to trans-activation through enriched expression of E2F1. (C) 2011 Elsevier B.V. All rights reserved.