Lentiviral Vectors and Adeno-Associated Virus Vectors: Useful Tools for Gene Transfer in Pain Research.
Lentiviral Vectors and Adeno-Associated Virus Vectors: Useful Tools for Gene Transfer in Pain Research.
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慢病毒载体和腺相关病毒载体：疼痛研究中基因转移的有用工具。
DOI：
10.1002/ar.23723
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发表时间：
2018-05
期刊：
影响因子：
--
通讯作者：
Wang YY
中科院分区：
文献类型：
--
作者：
Zheng CX;Wang SM;Bai YH;Luo TT;Wang JQ;Dai CQ;Guo BL;Luo SC;Wang DH;Yang YL;Wang YY
疼痛，特别是慢性疼痛，给个体和社会带来了沉重的负担，一直是基础和临床研究的热点。深入了解生物分子和各种离子通道在疼痛中的作用，可以揭示疼痛的机制，促进疼痛治疗的发展。利用病毒载体在靶位点转移特异性基因是一种有前途的研究和临床应用方法。慢病毒载体和腺相关病毒（AAV）载体允许转基因在非分裂细胞中稳定和长期表达，广泛应用于疼痛研究。本文对慢病毒载体和腺相关病毒载体的结构、分类、优缺点及递送方法进行了综述。慢病毒和腺相关病毒载体输送到靶点的方法与转基因表达的位点、水平和时期密切相关。重点放在各种递送方法适用于将载体传递到脊髓和背根神经节，这两个都在初级伤害性感受中发挥重要作用。我们的目标是提供洞察这两种病毒载体的特点，以及不同的疼痛研究可以选择哪种给药方式。Anat Rec，301：825-836，2018。版权所有© 2017作者。The Anatomical Record由Wiley Periodicals，Inc.出版。代表美国解剖学家协会
Pain, especially chronic pain, has always been a heated point in both basic and clinical researches since it puts heavy burdens on both individuals and the whole society. A better understanding of the role of biological molecules and various ionic channels involved in pain can shed light on the mechanism under pain and advocate the development of pain management. Using viral vectors to transfer specific genes at targeted sites is a promising method for both research and clinical applications. Lentiviral vectors and adeno‐associated virus (AAV) vectors which allow stable and long‐term expression of transgene in non‐dividing cells are widely applied in pain research. In this review, we thoroughly outline the structure, category, advantages and disadvantages and the delivery methods of lentiviral and AAV vectors. The methods through which lentiviral and AAV vectors are delivered to targeted sites are closely related with the sites, level and period of transgene expression. Focus is placed on the various delivery methods applied to deliver vectors to spinal cord and dorsal root ganglion both of which play important roles in primary nociception. Our goal is to provide insight into the features of these two viral vectors and which administration approach can be chosen for different pain researches. Anat Rec, 301:825–836, 2018. © 2017 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.
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