ACTIVE SITE AND MECHANISM OF ACTION OF BOVINE PANCREATIC RIBONUCLEASE
ACTIVE SITE AND MECHANISM OF ACTION OF BOVINE PANCREATIC RIBONUCLEASE
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DOI：
10.1038/190781a0
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发表时间：
1961-01-01
期刊：
影响因子：
64.8
通讯作者：
MATHIAS, AP
中科院分区：
文献类型：
--
作者：
FINDLAY, D;HERRIES, DG;MATHIAS, AP
获得的常数值如表 1 所示。在解释诸如此类的结果时必须严格保留”，不过，作为第一个近似值，可以将方程 (2) 和 (3) 的酸解离常数与反应方案的酸解离常数等同起来。K 1 和 K 的值表明两者都是从组氨酸残基的咪唑基团中解离。其中一个需要碱形式，另一个需要酸形式。测量的常数是“宏观”的；但是，由于它们的间距相对较大，它们可能与酸碱位点的固有 pK 值没有太大差异。
The values obtained for the constants are shown in Table 1. Serious reservations have to be made in interpreting results such as these", though, as a first approximation, it is permissible to identify the acid dissociation constants of equations (2) and (3) with those of the reaction scheme. The values of K, and K, suggest that both are dissociations from imidazole groups of histidine residues. One of these is required in the base form and the other in the acid form for catalytic activity. The measured constants are “macroscopic'º; but, because of their relatively large separation, they probably do not differ greatly from the intrinsic pK values of the acid and base sites.