Cordyceps militaris improves the survival of Dahl salt-sensitive hypertensive rats possibly via influences of mitochondria and autophagy functions.
Cordyceps militaris improves the survival of Dahl salt-sensitive hypertensive rats possibly via influences of mitochondria and autophagy functions.
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DOI：
10.1016/j.heliyon.2017.e00462
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发表时间：
2017-11
期刊：
影响因子：
4
通讯作者：
Gotow T
中科院分区：
文献类型：
--
作者：
Takakura K;Ito S;Sonoda J;Tabata K;Shiozaki M;Nagai K;Shibata M;Koike M;Uchiyama Y;Gotow T
虫草属及其特有成分虫草素因其多种健康益处和延长寿命的期望而备受关注。我们分析了含有大量虫草素的蛹虫草（CM）是否可以延长Dahl盐敏感大鼠的生存期，其生存期通过高盐饮食减少到103个月。这些寿命缩短的大鼠的生存期显着延长时，补充CM，可能是由于中风的影响最小化。其次，我们分析了CM对高血压敏感器官、中枢神经系统（CNS）、心脏、肾脏和肝脏的影响。我们试图确定CM如何改善器官，我们特别关注线粒体和自噬功能。以下结果来自CM治疗大鼠与对照大鼠的比较。显微镜下，CNS神经元、心肌细胞、肾小球足细胞、肾上皮细胞和肝细胞均得到改善。免疫印迹和免疫组织化学分析显示，在CNS神经元中，ATP合成酶β亚基、SIRT 3和SOD 2、自噬相关蛋白LC 3-II/LC 3-I比值和组织蛋白酶D的表达均显著降低，而在其他三个器官的细胞中则显著升高，而p62的表达在所有被测器官中均降低。在中枢神经系统中Akt和mTOR的活性增强，而AMPK的活性降低，而在其他器官中这种激酶活性则完全相反。总之，CM的影响可能在两个器官组之间的线粒体和自噬功能之间存在差异，因为在CNS中，线粒体和自噬似乎分别被抑制和促进，而在其他器官组中，线粒体和自噬都被激活。这可能与两个器官中稳定或改善的细胞活性有关，这可能导致这些大鼠的寿命延长。
The genus Cordyceps and its specific ingredient, cordycepin, have attracted much attention for multiple health benefits and expectations for lifespan extension. We analyzed whether Cordyceps militaris (CM), which contains large amounts of cordycepin, can extend the survival of Dahl salt-sensitive rats, whose survival was reduced to ∼3 months via a high-salt diet. The survival of these life-shortened rats was extended significantly when supplemented with CM, possibly due to a minimization of the effects of stroke. Next, we analyzed the effect of CM on hypertension-sensitive organs, the central nervous systems (CNS), heart, kidney and liver of these rats. We attempted to ascertain how the organs were improved by CM, and we paid particular attention to mitochondria and autophagy functions. The following results were from CM-treated rats in comparison with control rats. Microscopically, CNS neurons, cardiomyocytes, glomerular podocytes, renal epithelial cells, and hepatocytes all were improved. However, immunoblot and immunohistochemical analysis showed that the expressions of mitochondria-related proteins, ATP synthase β subunit, SIRT3 and SOD2, and autophagy-related proteins, LC3-II/LC3-I ratio and cathepsin D all were reduced significantly in the CNS neurons, but increased significantly in the cells of the other three organs, although p62 was decreased in its expression in all the organs tested. Activity of Akt and mTOR was enhanced but that of AMPK was reduced in the CNS, while such kinase activity was completely the opposite in the other organs. Together, the influence of CM may differ between mitochondria and autophagy functioned between the two organ groups, as mitochondria and autophagy seemed to be repressed and promoted, respectively, in the CNS, while both mitochondria and autophagy were activated in the others. This could possibly be related to the steady or improved cellular activity in both the organs, which might result in the life extension of these rats.