Expression of retinoid nuclear receptor superfamily members in human hair follicles and its implication in hair growth.
Expression of retinoid nuclear receptor superfamily members in human hair follicles and its implication in hair growth.
复制标题
类维生素A核受体超家族成员在人毛囊中的表达及其对毛发生长的影响。
DOI：
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发表时间：
1997
影响因子：
3.6
通讯作者：
B. Bernard
中科院分区：
文献类型：
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作者：
N. Billoni;B. Gautier;Y. Mahé;B. Bernard
由于临床证据的脱发和头发脱色后etretinate治疗已被报道，我们决定研究的几个成员的维甲酸核受体超家族在真皮和上皮细胞的人毛囊的表达水平。此外，我们评估了这些受体的几种配体对体外培养的人毛发生长的影响。我们观察到，细胞/细胞质视黄酸（RA）结合蛋白-II和类维生素A-X-受体-α不断和强烈表达在两个车厢的维生素D受体的水平相当。在毛乳头细胞中，与总是表达的RAR β相反，RAR α和RAR γ并不恒定表达。在真皮鞘成纤维细胞中，RAR α、RAR β和RAR γ mRNA均适度表达，而在上皮隔室中，即拔出的毛发中，我们观察到在RAR β不存在的情况下相同基因的表达。我们还观察到RAR激动剂全反式RA和CD 367抑制体外培养的人毛囊的存活，而RXR激动剂CD 2425刺激毛发生长和存活的水平与1 α，25-二羟基维生素D3相当，这表明RXR激动剂可能在体内刺激人类毛发生长。
Since clinical evidence of hair loss and hair depigmentation following etretinate therapy has been reported, we decided to study the expression levels of several members of the retinoid nuclear receptor superfamily in dermal and epithelial compartments of the human hair follicle. Additionally, we evaluated the effects of several ligands for these receptors on human hair growth in culture in vitro. We observed that the cellular/ cytoplasmic retinoic acid (RA) binding protein-II and the retinoid-X-receptor-alpha were constantly and strongly expressed in both compartments at levels comparable to those of vitamin D receptor. In dermal papilla cells, by contrast with RAR beta which was always expressed, RAR alpha and RAR gamma were not constantly expressed. In dermal sheath fibroblasts, both RAR alpha, RAR beta and RAR gamma mRNAs were moderately expressed, while in the epithelial compartment, namely the plucked hair, we observed the expression of the same genes in the absence of RAR beta. We also observed that RAR agonists all-trans RA and CD367 inhibited the survival of human hair follicles in culture in vitro, while RXR agonist CD2425 stimulated hair growth and survival at levels comparable to those of 1 alpha,25-dihydroxyvitamin D3, suggesting that RXR agonists might stimulate hair growth in humans in vivo.