Microbial competition between Bacillus subtilis and Staphylococcus aureus monitored by imaging mass spectrometry
Microbial competition between Bacillus subtilis and Staphylococcus aureus monitored by imaging mass spectrometry
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DOI：
10.1099/mic.0.048736-0
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发表时间：
2011-09-01
期刊：
影响因子：
2.8
通讯作者：
Dorrestein, Pieter C.
中科院分区：
文献类型：
--
作者：
Gonzalez, David J.;Haste, Nina M.;Dorrestein, Pieter C.
微生物竞争存在于一般环境中，例如单细胞或多细胞真核生命形式之上或之内的土壤或水生生境。控制微生物竞争、导致生态位建立和微生物垄断的分子行为仍未确定。新兴的成像质谱（IMS）技术使人们能够观察到，当枯草芽孢杆菌与金黄色葡萄球菌共培养时，其代谢输出具有方向性。定向释放的抗生素改变金黄色葡萄球菌毒力因子的产生和定植。因此，IMS 提供了对竞争性微生物遭遇和生态位建立的很大程度上隐藏的本质的洞察，并为未来的抗生素发现提供了范例。
Microbial competition exists in the general environment, such as soil or aquatic habitats, upon or within unicellular or multicellular eukaryotic life forms. The molecular actions that govern microbial competition, leading to niche establishment and microbial monopolization, remain undetermined. The emerging technology of imaging mass spectrometry (IMS) enabled the observation that there is directionality in the metabolic output of the organism Bacillus subtilis when co-cultured with Staphylococcus aureus. The directionally released antibiotic alters S. aureus virulence factor production and colonization. Therefore, IMS provides insight into the largely hidden nature of competitive microbial encounters and niche establishment, and provides a paradigm for future antibiotic discovery.