Role of epithelial interleukin-8 (IL-8) and neutrophil IL-8 receptor A in Escherichia coli-induced transuroepithelial neutrophil migration
Role of epithelial interleukin-8 (IL-8) and neutrophil IL-8 receptor A in Escherichia coli-induced transuroepithelial neutrophil migration
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DOI：
10.1128/iai.65.8.3451-3456.1997
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发表时间：
1997-08-01
影响因子：
3.1
通讯作者：
Agace, WW
中科院分区：
文献类型：
--
作者：
Godaly, G;Proudfoot, AEI;Agace, WW
大肠杆菌刺激中性粒细胞跨人类尿路上皮细胞层迁移，本研究调查了中性粒细胞趋化因子白细胞介素-8 (IL-8)在此过程中的作用，大肠杆菌和IL-1a刺激尿道上皮层分泌IL-8并诱导跨上皮中性粒细胞迁移，抗IL-8抗体减少中性粒细胞跨上皮细胞层迁移，表明该趋化因子在迁移过程中的核心作用，此外，将重组IL-8添加至未刺激的细胞层足以诱导迁移。通过洗涤细胞层去除可溶性IL-8后，嗜中性粒细胞迁移的IL-8依赖性得以维持。使用异硫氰酸荧光素标记的IL-8进行流式细胞术分析证实了IL-8与上皮细胞表面结合的能力，利用共焦激光扫描显微镜进行的间接免疫荧光显示IL-8与上皮细胞层结合。先前将中性粒细胞与 IL-8 受体 A (IL-8RA) 抗体一起孵育可减少中性粒细胞迁移，抗 IL-8 RE 抗体对中性粒细胞迁移没有影响。这些结果表明，IL-8 在大肠杆菌或 IL-1 α 诱导的跨尿上皮迁移中发挥关键作用，并表明上皮细胞产生的 IL-8 与中性粒细胞表面上的 IL-8RA 相互作用。
Escherichia coli stimulates neutrophil migration across human uroepithelial cell layers, This study investigated the role of the neutrophil chemokine interleukin-8 (IL-8) in this process, E. coli and IL-la stimulated urinary tract epithelial layers to secrete IL-8 and induced transepithelial neutrophil migration, Anti-IL-8 antibody reduced neutrophil migration across epithelial cell layers, indicating a central role for this chemokine in the migration process, Furthermore, addition of recombinant IL-8 to unstimulated cell layers was sufficient to induce migration, The IL-8 dependence of neutrophil migration was maintained after removal of soluble IL-8 by washing of the cell layers. Flow cytometry analysis with fluorescein isothiocyanate-labelled IL-8 confirmed IL-8's ability to bind to the epithelial cell surface, Indirect immunofluorescence with confocal laser scanning microscopy showed that IL-8 associated with the epithelial cell layers. Prior incubation of neutrophils with antibodies to IL-8 receptor A (IL-8RA) reduced neutrophil migration, Anti-IL-8 RE antibody had no effect on neutrophil migration, These results demonstrate that IL-8 plays a key role in E. coli- or IL-1 alpha-induced transuroepithelial migration and suggest that epithelial cell-produced IL-8 interacts with IL-8RA on the neutrophil surface.