Isolation of a virus infecting the novel shellfish- killing dinoflagellate Heterocapsa circularisquama
Isolation of a virus infecting the novel shellfish- killing dinoflagellate Heterocapsa circularisquama
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感染新型甲壳类甲藻Heterocapsacircularisquama的病毒的分离
DOI：
10.3354/ame023103
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发表时间：
2001
影响因子：
1.4
通讯作者：
M. Yamaguchi
中科院分区：
文献类型：
--
作者：
K. Tarutani;Keizo Nagasaki;S. Itakura;M. Yamaguchi
1999年8月，在日本近海发生的一次环状甲藻水华期间，从日本近海分离到一种新的杀死甲壳类的环状异鞭毛藻病毒(H.Circularisquama，简称：丙型肝炎)。超薄切片的透射电子显微镜显示，感染后24~48h，细胞内可见病毒样颗粒。病毒为二十面体，无尾巴，直径180~210 nm(平均±标准差=197±8 nm)，含有电子致密核。这是一种双链DNA病毒，病毒颗粒的出现与细胞质中没有出现在未感染细胞内的颗粒区(病毒质)有关。该病毒可引起日本中西部不同海湾分离的18株环状螺旋藻的细胞裂解，但不能裂解其他24种浮游植物。据我们所知，这是首次报道一种感染甲藻的病毒，该病毒已被分离并在培养中保持，我们的结果表明，感染并导致甲藻裂解的病毒是天然海洋病毒群落的组成部分。
A virus infecting the novel shellfish-killing dinoflagellate Heterocapsa circularisquama (H. circularisquama Virus: HcV) was isolated from Japanese coastal waters in August 1999 during a H. circularisquama bloom. Transmission electron microscopy of ultrathin sections of infected H. cir- cularisquama revealed the presence of intracellular virus-like particles 24 to 48 h after infection. The virus was icosahedral, lacking a tail, ca 180 to 210 nm (mean ± standard deviation = 197 ± 8 nm) in diameter and contained an electron-dense core. It was a double-stranded DNA virus, and the appear- ance of the virus particles was associated with a granular region (viroplasm) in the cytoplasm that did not appear within uninfected cells. The virus caused cell lysis of 18 strains of H. circularisquama iso- lated from various embayments throughout central and western Japan, but did not lyse 24 other phytoplankton species that were tested. To our knowledge, this is the first report of a virus infecting dinoflagellates which has been isolated and maintained in culture, and our results demonstrate that viruses which infect and cause lysis of dinoflagellates are a component of natural marine viral com- munities.