Identification of the Chromatin Regions Coated by Non-coding Xist RNA
Identification of the Chromatin Regions Coated by Non-coding Xist RNA
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DOI：
10.1159/000207514
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发表时间：
2009-07
影响因子：
1.7
通讯作者：
K. Murakami;T. Ohhira;E. Oshiro;D. Qi;M. Oshimura;H. Kugoh
中科院分区：
文献类型：
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作者：
K. Murakami;T. Ohhira;E. Oshiro;D. Qi;M. Oshimura;H. Kugoh
存在的非编码RNA （ncRNA）是X染色体失活（XCI）的关键。一些基因可以从XCI中逃脱，但这是如何发生的尚不清楚。我们建立了一种改良的RNA标记和相关蛋白恢复（TRAP）方法来研究Xist RNA与其靶基因之间的关联。在小鼠细胞中，在Uba1基因上检测到Xist RNA，但在Jarid1c和Utx基因上检测不到Xist RNA，这些基因从XCI中逃逸。使用这种技术，我们能够证明Xist RNA分子不存在于从XCI逃逸的活性基因上，但存在于被XCI灭活的基因上，这表明该方法是ncRNA功能分析的有力工具。
Xist non-coding RNA (ncRNA) is essential for X chromosome inactivation (XCI). Some genes can escape from XCI, but how this occurs is unknown. We developed a modified RNA tagging and recovery of associated proteins (TRAP) method to study the association between Xist RNA and its target genes. In mouse cells, Xist RNA was detected on the Uba1 gene, but not on Jarid1c and Utx genes, which escape from XCI. Using this technique we were able to show that the Xist RNA molecule is not present on active genes that escape from XCI, but is present on genes inactivated by XCI, suggesting that this method is a powerful tool for functional analysis of ncRNA.