Differential expression of fatty acid transport proteins in epidermis and skin appendages
Differential expression of fatty acid transport proteins in epidermis and skin appendages
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DOI：
10.1111/j.0022-202x.2005.23934.x
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发表时间：
2005-12-01
影响因子：
6.5
通讯作者：
Stahl, A
中科院分区：
文献类型：
--
作者：
Schmuth, M;Ortegon, AM;Stahl, A
表皮和皮脂细胞来源的脂类既来自新生合成，也来自循环中的脂肪酸吸收。质膜蛋白在后一过程中起着重要作用。特别是，脂肪酸运输蛋白(FATP/溶质载体家族27)是一种完整的跨膜蛋白，可以增强细胞对长链脂肪酸的摄取。使用针对所有六种哺乳动物FATP的特异性抗血清，我们发现人和小鼠的皮肤都表达FATP1、-3、-4和-6。在成人皮肤中，FATP1和FATP3主要由角质形成细胞表达，而FATP4在皮脂腺和FATP6在毛囊上皮细胞中强烈表达。持续的屏障破坏导致FATP1和-6水平的增加以及CD36蛋白的强劲增加。值得注意的是，与成体表皮相比，胚胎角质形成细胞在18.5天的FATP1表达较低，而FATP4表达较高。综上所述，这些发现表明，FATP不仅在皮肤内由不同类型的细胞表达，而且它们的定位在发育过程中受到动态调节。
Epidermis and sebocyte-derived lipids are derived both from de novo synthesis and through uptake of fatty acids from the circulation. Plasma membrane proteins can significantly contribute to the latter process. In particular, fatty acid transport proteins (FATP/solute carrier family 27) are integral transmembrane proteins that enhance the uptake of long-chain fatty acids into cells. Using specific antisera against all six mammalian FATP, we found that both human and mouse skin express FATP1, -3,-4, and -6. In adult skin, FATP1 and -3 are expressed predominantly by keratinocytes, whereas FATP4 is strongly expressed by sebaceous glands and FATP6 by hair follicle epithelia. Sustained barrier disruption leads to increases in FATP1 and -6 levels as well as a robust increase in CD36 protein. Notably, expression of FATP1 by embryonic keratinocytes at day 18.5 was lower, and FATP4 increased in comparison with adult epidermis. Together, these findings indicate that FATP are not only expressed by different cell types within the skin, but also that their localization is dynamically regulated during development.