Increased tryptophan hydroxylase activity in serotonergic nerve terminals spared by 5,7-dihydroxytryptamine.
Increased tryptophan hydroxylase activity in serotonergic nerve terminals spared by 5,7-dihydroxytryptamine.
复制标题
5,7-二羟色胺可增加血清素能神经末梢中色氨酸羟化酶的活性。
DOI：
10.1016/0006-2952(86)90266-2
复制
发表时间：
1986
影响因子：
5.8
通讯作者：
Zigmond,MJ
中科院分区：
文献类型：
--
作者：
Stachowiak,MK;Stricker,EM;Jacoby,JH;Zigmond,MJ
成年大鼠接受脑室内注射 5,7-二羟色胺 (5,7-DHT)，以破坏整个大脑中含有血清素 (5-HT) 的神经末梢。当3或21天后处死动物时，我们观察到隔膜和海马中的5-HT含量显着下降，并且体外高亲和力5-HT摄取也平行下降。 5-羟基吲哚乙酸 (5-HIAA) 浓度也有所降低，但幅度要小得多，导致 5-HIAA 与 5-HT 的比率显着增加。 These changes were accompanied by similar increases in the ratio of tryptophan hydroxylase (TPH) activity to 5-HT content. TPH 活性的相对增加是由两个时间上不同的过程引起的，第一个过程似乎是可以通过 Ca2+ 依赖性磷酸化在体外模拟的激活。我们的结论是，在 5-HT 神经元部分受损后，剩余末端的 5-HT 合成和释放会出现代偿性增加。
Adult rats received intraventricular injections of 5,7-dihydroxytryptamine (5,7-DHT) to destroy serotonin (5-HT)-containing nerve terminals throughout the brain. When the animals were killed 3 or 21 days later, we observed a marked decrease in 5-HT content in septum and hippocampus and a parallel decline inin vitrohigh affinity 5-HT uptake. 5-Hydroxyindoleacetic acid (5-HIAA) concentrations also were reduced but by a much smaller extent, resulting in significant increases in the ratio of 5-HIAA to 5-HT. These changes were accompanied by similar increases in the ratio of tryptophan hydroxylase (TPH) activity to 5-HT content. The relative increases in TPH activity resulted from two temporally distinct processes, the first of which appeared to be an activation that could be mimickedin vitroby Ca2+-dependent phosphorylation. We conclude that, after partial damage to 5-HT neurons, there is a compensatory increase in the synthesis and release of 5-HT from those terminals that remain.