Primary genetic linkage maps of the ascidian, Ciona intestinalis
Primary genetic linkage maps of the ascidian, Ciona intestinalis
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DOI：
10.2108/zsj.23.31
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发表时间：
2006-01-01
影响因子：
0.9
通讯作者：
Sordino, Paolo
中科院分区：
文献类型：
--
作者：
Kano, Shungo;Satoh, Nori;Sordino, Paolo
为了进行原脊索动物的全基因组分析，我们利用F1代假测交作图策略和扩增片段长度多态性（AFLP）标记构建了海鞘被囊类玻璃海鞘的初级连锁图谱。两个遗传图谱包括14个连锁群，在协议的单倍体染色体数目，并包含276和125个AFLP位点来自英国和那不勒斯之间的个人杂交。这两张地图的覆盖范围分别为4218.9和2086.9 cM，平均标记间距分别为16.1和18.9 cM。我们观察到一个高的重组率，范围从25至49 kb/cM，这可以解释在这个物种的高度多态性。通过序列测定将部分AFLP标记转化为序列标记位点（STS），以建立片段物理图谱的锚标记。我们的重组工具提供了遗传状态和全基因组组织的基本知识，以及遗传标记，以协助C。肠的。
For whole-genome analysis in a basal chordate (protochordate), we used F1 pseudo-testcross mapping strategy and amplified fragment length polymorphism (AFLP) markers to construct primary linkage maps of the ascidian tunicate Ciona intestinalis. Two genetic maps consisted of 14 linkage groups, in agreement with the haploid chromosome number, and contained 276 and 125 AFLP loci derived from crosses between British and Neapolitan individuals. The two maps covered 4218.9 and 2086.9 cM, respectively, with an average marker interval of 16.1 and 18.9 cM. We observed a high recombinant ratio, ranging from 25 to 49 kb/cM, which can explain the high degree of polymorphism in this species. Some AFLP markers were converted to sequence tagged sites (STSs) by sequence determination, in order to create anchor markers for the fragmental physical map. Our recombination tools provide basic knowledge of genetic status and whole genome organization, and genetic markers to assist positional cloning in C. intestinalis.