Culturable fungal assemblages growing within Cenococcum sclerotia in forest soils
Culturable fungal assemblages growing within Cenococcum sclerotia in forest soils
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森林土壤中新球菌核内生长的可培养真菌群落
DOI：
10.1111/1574-6941.12428
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发表时间：
2014
影响因子：
4.2
通讯作者：
Smith M
中科院分区：
文献类型：
--
作者：
Obase K;Douhan G;Matsuda Y;Smith M
外生菌根真菌cenococum geophilum（子囊菌门，dothideomyctes）在森林土壤中形成黑色，圆形至不规则的菌核。定植在菌核上的真菌似乎会影响菌核的活力，并可能在新球菌的生活史中发挥重要作用。一些真菌还可以通过分解新球藻来影响营养循环，新球藻是黑化的，不易腐烂。我们使用了一种基于培养的方法来记录真菌群落生长在从森林土壤中收集的表面消毒的菌核内。971个菌核中有297个菌核成功分离到新球菌，而427个菌核中存在非新球菌的真菌。对内部转录的间隔序列rDNA进行DNA条形码，然后按97%的序列相似性分组，得到85个操作分类单位（otu），主要由子囊菌组成(例如；毛囊菌门、euroti门、heloti门、pleospor门)和少量担子菌门和毛菌门。虽然大多数真菌OTUs很少被培养，但一些真菌OTUs，如asterostroma，Cladophialophora， oididendron和pleosporales的成员是常见的，并且在许多地点都能发现。我们的研究结果表明，新球菌菌核作为多种真菌的底物。在许多地点的菌核中出现了几个otu，这表明这些真菌可能是新球菌菌核的活性寄生虫，或者可能优先利用菌核作为营养来源。
The ectomycorrhizal fungusCenococcum geophilum(Ascomycota,Dothideomycetes) forms black, round to irregular sclerotia in forest soils. Fungi that colonize the sclerotia appear to affect sclerotia viability and may play an important role in the life history ofCenococcum. Some of the fungi could also affect nutrient cycling by decomposingCenococcumsclerotia, which are melanized and recalcitrant to decay. We used a culture-based method to document the fungal communities growing inside surface-sterilized sclerotia that were collected from forest soils.Cenococcumwas successfully isolated from 297 of 971 sclerotia whereas 427 sclerotia hosted fungi other thanCenococcum. DNA barcoding of the internal transcribed spacer rDNA followed by grouping at 97% sequence similarity yielded 85 operational taxonomic units (OTUs) that consisted primarily ofAscomycota(e.g.Chaetothyriales,Eurotiales,Helotiales,Pleosporales) and a fewBasidiomycotaandMucoromycotina. Although most fungal OTUs were infrequently cultured, several OTUs such as members ofAsterostroma,Cladophialophora,Oidiodendron,andPleosporaleswere common and found across many sites. Our results suggest thatCenococcumsclerotia act as a substrate for diverse fungi. The occurrence of several OTUs in sclerotia across many sites suggests that these fungi may be active parasites ofCenococcumsclerotia or may preferentially use sclerotia as a nutrient source.