Hyperinsulinemia in tube-fed hypophysectomized rats bearing hypothalamic lesions.
Hyperinsulinemia in tube-fed hypophysectomized rats bearing hypothalamic lesions.
复制标题
具有下丘脑损伤的管饲垂体切除大鼠的高胰岛素血症。
DOI：
10.1152/ajplegacy.1970.219.6.1632
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
L. Frohman
中科院分区：
文献类型：
--
作者：
P. W. Han;L. Frohman
材料和方法研究对象为芝加哥荷尔蒙分析实验室提供的雄性HYPERX大鼠。当大鼠体重200g，大约50日龄时，进行脑下叶切除。他们被供应商保存了IO术后(PO)天，然后才送到我们这里。到达后，它们被关在单独的笼子里，并提供食物(普里纳实验室的食物)和随意的水。L在整个研究过程中每周测量三次个人体重。下丘脑手术前3周体重增加超过10g的大鼠被认为是不完全切除的，被丢弃。在放大镜下检查鞍区和脑底的垂体残留物时，在尸检时检查垂体切除的完整性。所有纳入本报告的大鼠都接受了完整的脑垂体切除手术。在己巴比妥钠麻醉(6rng/100g，ip)下，双侧下丘脑病变位于
MATERIALS AND METHODSThirty-one male hypex rats purchased from the Hormone Assay Laboratory of Chicago were studied. Hypophysectomies were performed when the rats weighed 200 g and were approximateIy 50 days old. They were kept by the supplier for IO postoperative(PO) days before being sent to us. After their arrival, they were housed in individual cages and supplied with food (Purina laboratory chow) and water ad libitum. Individual body weight was measured l-3 times a week throughout the course of this study. Those rats which gained more than 10 g in the 3 weeks prior to hypothalamic operation were considered incompletely hypophysectomized and were discarded. The completeness of the hypophysectomy was checked at autopsy when the sella turcica and the base of the brain were examined for pituitary remnants under a magnifying glass. All rats included in this report had a complete hypophysectomy. Under sodium hexobarbital anesthesia (6 rng/lOO g, ip), bilateral hypothalamic lesions were placed in the region of