Immunoglobulin JH, C mu, and C gamma gene rearrangements in human B lymphocytes clonally transformed by Epstein-Barr virus.
Immunoglobulin JH, C mu, and C gamma gene rearrangements in human B lymphocytes clonally transformed by Epstein-Barr virus.
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Epstein-Barr 病毒克隆转化的人 B 淋巴细胞中免疫球蛋白 JH、C mu 和 C gamma 基因重排。
DOI：
10.1073/pnas.82.2.556
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发表时间：
1985
影响因子：
11.1
通讯作者：
Calame,KL
中科院分区：
文献类型：
--
作者：
Brown,NA;Liu,C;Berenson,JR;Garcia,CR;Wang,R;Calame,KL
免疫球蛋白基因片段的体细胞重排和缺失已在几种类型的鼠B细胞中得到证实。此外，在人前B细胞白血病和B细胞淋巴瘤中已经报道了JH、C μ和轻链免疫球蛋白基因片段的重排。我们已经使用重组DNA探针的人JH，C μ，和C γ免疫球蛋白基因座分析与重链基因表达在人B细胞克隆转化EB病毒的遗传事件。BamHI消化的细胞克隆DNA的Southern杂交分析显示，这些人B细胞克隆通常具有双等位基因JH重排，并且重链同种型转换与双染色体C μ和C γ基因重排相关。观察到生殖系C μ和C γ片段的缺失，有时是双等位基因。总体而言，观察到的重链恒定区基因的重排和缺失表明，人重链类别转换以与沿14号染色体沿着的重链基因类别的拟定顺序一致的一般顺序进行。
Somatic rearrangements and deletions of immunoglobulin gene segments have been demonstrated in several types of murine B cells. In addition, rearrangements of the JH, C mu, and light chain immunoglobulin gene segments have been reported in human pre-B-cell leukemias and B-cell lymphomas. We have used recombinant DNA probes for the human JH, C mu, and C gamma immunoglobulin gene loci to analyze the genetic events associated with heavy chain gene expression in human B cells clonally transformed by Epstein-Barr virus. Southern hybridization analysis of BamHI-digested cell clone DNAs shows that these human B-cell clones often have bi-allelic JH rearrangements and that heavy chain isotype switching is associated with bichromosomal C mu and C gamma gene rearrangements. Deletions of germ line C mu and C gamma segments were observed that were sometimes bi-allelic. Overall, the observed rearrangements and deletions of heavy chain constant region genes suggest that human heavy chain class switching proceeds in a general order consistent with the proposed order of the heavy chain gene classes along chromosome 14.