Identification and partial characterization of hepatocyte-stimulating factor from leukemia cell lines: comparison with interleukin 1.
Identification and partial characterization of hepatocyte-stimulating factor from leukemia cell lines: comparison with interleukin 1.
复制标题
白血病细胞系肝细胞刺激因子的鉴定和部分表征：与白细胞介素 1 的比较。
DOI：
10.1073/pnas.82.5.1443
复制
发表时间：
1985
影响因子：
11.1
通讯作者：
Fuller,GM
中科院分区：
文献类型：
--
作者：
Woloski,BM;Fuller,GM
单核细胞系白血病细胞系(HL-60、U-937和P388D1)在佛波二酯诱导分化后产生一种肝细胞刺激因子(HSF)。在24-72小时内，这些白血病细胞产生的HSF量是人类外周血单核细胞的2-30%。分化早期的系列细胞产生更多的HSF。用高效液相色谱法对每种细胞类型的培养液进行分级，发现HSF的大部分活性在25千到30千道尔顿范围内。在相同的培养条件下，可以产生白介素1；然而，其生物活性在7千到15千道尔顿范围内。这两种单核因子都没有表现出相互的生物活性。大量增加白细胞介素1产量的超诱导培养条件完全消除了HSF的产生，这表明每种蛋白质编码的mRNA的稳定性不同，或者在诱导这些蛋白质合成方面存在不同的时间事件。
Leukemia cell lines of the monocytic series (HL-60, U-937, and P388D1) produce a hepatocyte-stimulating factor (HSF) following induction of differentiation with phorbol diester. In 24-72 hr, these leukemia cells produce 2-30% the amount of HSF as human peripheral blood monocytes. Cells of the series at earlier stages of differentiation produced greater amounts of HSF. Fractionation of the medium from each cell type by HPLC reveals much of the HSF activity in the 25- to 30-kilodalton range. Under the same culture conditions, interleukin 1 is produced; however, its bioactivity is in the 7- to 15-kilodalton range. Neither monokine shows reciprocal bioactivity. Superinducing culture conditions that greatly increase interleukin 1 production completely eliminate HSF production, suggesting that there is different stability of the mRNA coding for each protein or that there are different temporal events important to the induction of synthesis of these proteins.