Electrocatalytic oxidation of the reduced nicotinamide adenine dinucleotide at carbon ionic liquid electrode modified with polythionine/multi-walled carbon nanotubes composite
Electrocatalytic oxidation of the reduced nicotinamide adenine dinucleotide at carbon ionic liquid electrode modified with polythionine/multi-walled carbon nanotubes composite
复制标题
DOI：
10.1007/s00604-009-0285-5
复制
发表时间：
2010-01
影响因子：
5.7
通讯作者：
N. Mai;Xiaoying Liu;Xiandong Zeng;Liang Xing;Wanzhi. Wei;S. Luo
中科院分区：
文献类型：
--
作者：
N. Mai;Xiaoying Liu;Xiandong Zeng;Liang Xing;Wanzhi. Wei;S. Luo
采用聚硫氨酸(PTh)/多壁碳纳米管（MWCNTs）复合材料修饰碳离子液体电极（CILE），用于检测还原性烟酰胺腺嘌呤二核苷酸（NADH）。在中性介质中，将硫氨酸电化学聚合在MWCNTs上制备电极。循环伏安法表明，该电极能在低至0.03 V的过电位下介导NADH的氧化。安培实验表明，在5 s内对NADH产生了敏感和稳定的响应。测定NADH的线性范围为0.8 ~ 422 μmol L−1，检出限为0.26 μmol L−1（S/N = 3）。该方法线性范围宽，检测限低，对NADH的响应速度快，这表明该方法可用于开发基于NAD+依赖性酶的生物传感器。
A carbon ionic liquid electrode (CILE) was modified with a polythionine (PTh)/multi-walled carbon nanotubes (MWCNTs) composite and used for the detection of reduced nicotinamide adenine dinucleotide (NADH). The electrode was prepared by electrochemical polymerization of thionine on the MWCNTs in neutral medium. Cyclic voltammetry indicated that the electrode was capable of mediating the oxidation of NADH at an overpotential as low as 0.03 V. Amperometric experiments showed that a sensitive and stable response towards NADH is obtained within 5 s. The linear range for the determination of NADH is from 0.8 μmol L−1to 422 μmol L−1, with a detection limit of 0.26 μmol L−1(S/N = 3). The wide linear range, lower detection limit and faster response towards NADH suggests that the new method potentially is useful for developing NAD+-dependent enzyme-based biosensors.