Enzyme-linked immunosorbent assay for A and B water soluble blood group substances.
Enzyme-linked immunosorbent assay for A and B water soluble blood group substances.
复制标题
A 和 B 水溶性血型物质的酶联免疫吸附测定。
DOI：
10.1520/jfs11856j
复制
发表时间：
1986
影响因子：
1.6
通讯作者：
J. Thorpe
中科院分区：
文献类型：
--
作者：
S. Bolton;J. Thorpe
本文研究了影响唾液血型物质酶联免疫吸附测定的条件。发现A、B和O分泌型唾液样品将各自结合抗A和抗B分型试剂。对影响试验响应的条件进行了优化，以获得最大灵敏度，并在与同源抗原结合的抗血清结果和异源抗原-抗体组合的响应之间获得最高分辨率。单克隆抗体消除了异源结合，表明这种结合是由于常规分型试剂缺乏特异性。描述了一种使用单克隆抗体区分A和B分泌唾液样品的灵敏测定。
The conditions affecting an enzyme-linked immunosorbent assay for salivary blood group substances were investigated. It was found that A, B, and O secretor saliva samples would each bind both anti-A and anti-B typing reagents. The conditions that affected the assay response were optimized for maximum sensitivity and to give the highest resolution possible between the result for an antiserum binding to homologous antigen and the response for heterologous antigen-antibody combinations. Monoclonal antibodies eliminated the heterologous binding indicating that this binding was due to a lack of specificity of the routine typing reagents. A sensitive assay using the monoclonal antibodies to distinguish between samples of A and B secretor saliva is described.