Characterization of a profilin-like protein from Schistosoma japonicum, a potential new vaccine candidate
Characterization of a profilin-like protein from Schistosoma japonicum, a potential new vaccine candidate
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DOI：
10.1007/s00436-008-0919-2
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发表时间：
2008-03
影响因子：
2
通讯作者：
Shuang-min Zhang;Z. Lv;He-jun Zhou;Li-yan Zhang;Lin-lin Yang;Xinbing Yu;Huanqin Zheng;Zhong-dao Wu
中科院分区：
文献类型：
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作者：
Shuang-min Zhang;Z. Lv;He-jun Zhou;Li-yan Zhang;Lin-lin Yang;Xinbing Yu;Huanqin Zheng;Zhong-dao Wu
扁形虫体被膜对调节宿主反应和寄生虫存活至关重要。经生物信息学分析，获得了一个含390 bp开放阅读框的互补脱氧核糖核酸（cDNA），编码一个类似profilin的蛋白质，其理论等电点为6.02，分子量为14.42 kDa。将该cDNA编码区克隆到原核表达载体pGEX-4 T-1中，在大肠杆菌中表达。纯化的重组蛋白rSj 15具有免疫原性，并能在小鼠体内激发出高滴度的抗体。Western blot分析表明，该蛋白在日本血吸虫不同生长阶段有差异表达，免疫组织化学分析表明，该蛋白定位于日本血吸虫皮层及下层组织。日本小虫rSj 15可诱导培养的小鼠树突状细胞表达IL-12。
The tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. A complementary deoxyribonucleic acid (cDNA) containing an open reading frame of 390 bp, which encodes a profilin-like tegumental protein with a theoretical isoelectric point of 6.02 and a molecular weight of 14.42 kDa, had been identified by bioinformatic analysis. The coding region of the cDNA was cloned into the prokaryotic expression vector pGEX-4T-1 and expressed inEscherichia coli. The purified recombinant protein named rSj15 was immunogenic and could elicit a high titer of antibody in mice. Western blot analysis revealed that the protein was differentially expressed during the different growth stages ofSchistosoma japonicum.Immunohistochemical analysis localized the protein to the tegument and underlying tissue of theS. japonicumadult worm. The rSj15 could induce the expressions of IL-12 in the cultured mouse dendritic cell.